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Research Database PMU-SQQUID

CD45-positive cells of haematopoietic origin enhance chondrogenic marker gene expression in rat marrow stromal cells.
Ahmed, N; Vogel, B; Rohde, E; Strunk, D; Grifka, J; Schulz, MB; Grassel, S
Int J Mol Med. 2006; 18(2):233-240
Full papers/articles (Journal)


Rohde Eva
Strunk Dirk


Adult mesenchymal stem cells (MSCs) can be readily isolated from bone marrow, expanded in culture and subsequently subjected to differentiation into various connective tissue lineages. In general, for animal studies separation of MSCs from other bone marrow-derived cells is achieved by sole adherence to plastic surface of tissue culture flasks; however, this procedure produces a heterogeneous cell population containing CD45-positive haematopoietic cells (HCs) and haematopoietic stem cells (HSCs). It is known, that mixed cell cultures consisting of cocultures of differentiated somatic cells with adult stem cells promote differentiation towards specific cell lineages. For determining the effect of the CD45-positive cell population on the differentiation potential of MSCs, we sorted out the bone marrow-derived adherent cells by immunomagnetic technique (MACS) to attain a subpopulation of CD45-depleted cells. Herein, we show that the presence of adherent CD45-positive HCs not only promote expression of the chondrogenic marker genes Col2a1, COMP and Sox9, but also of Col1a1, Col10a1 and to a certain degree Cbfa1 in MSCs when cultured in an appropriate three-dimensional environment. These observations constitute a step towards unravelling the influence of haematopoietic cells on chondrogenic differentiation of MSCs.

Useful keywords (using NLM MeSH Indexing)


Antigens, CD45/metabolism*


Bone Marrow Cells/cytology

Bone Marrow Cells/physiology*

Cell Culture Techniques

Cell Differentiation

Cells, Cultured


Gene Expression Profiling

Gene Expression Regulation*

Hematopoietic Stem Cells/cytology

Hematopoietic Stem Cells/physiology


Immunomagnetic Separation


Mesenchymal Stromal Cells/cytology

Mesenchymal Stromal Cells/physiology*

Molecular Sequence Data


Rats, Sprague-Dawley

Stromal Cells/cytology

Stromal Cells/physiology*

Find related publications in this database (Keywords)

rat marrow stromal cells
magnetic associated cell sorting
chondrogenic differentiation
haematopoietic cells