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Research Database PMU-SQQUID

An additional exon in the human vinculin gene specifically encodes meta-vinculin-specific difference peptide. Cross-species comparison reveals variable and conserved motifs in the meta-vinculin insert.
Koteliansky, VE; Ogryzko, EP; Zhidkova, NI; Weller, PA; Critchley, DR; Vancompernolle, K; Vandekerckhove, J; Strasser, P; Way, M; Gimona, M;
Eur J Biochem. 1992; 204(2): 767-772.
Originalarbeiten (Zeitschrift)

PMU-Authors

Gimona Mario
Strasser Peter

Abstract

We have analyzed the structure, origin and expression of the high-molecular-mass muscle-specific variant of vinculin, called meta-vinculin. The meta-vinculin-specific inserts from the human and avian molecules have been isolated and sequenced and the sequences confirmed via cloning of the corresponding cDNA. Comparison of the human, avian and determined porcine sequences revealed cross-species identity in the C-terminal half of the insert. Human and porcine meta-vinculin were highly similar in the insert region, showing only five amino acid exchanges; avian meta-vinculin showed 22 exchanges in the same region compared to human meta-vinculin and exhibited, in addition, one extra amino acid, making 69 in all. Each insert was flanked by characteristic KWSSK motifs. Evidence for two vinculin mRNA species in human uterus smooth muscle was provided by reverse transcription combined with the polymerase chain reaction, as well as by ribonuclease-mapping analysis of cDNA/mRNA hybrids. One of the mRNA species contained an additional 204-nucleotide insert that precisely encoded the meta-vinculin-specific peptide. Sequence analysis of the appropriate portion of the human vinculin gene showed that the section coding for the meta-vinculin-specific insert is present as a discrete exon. Thus, meta-vinculin and vinculin mRNA are generated by alternative splicing.


Useful keywords (using NLM MeSH Indexing)

Amino Acid Sequence

Animals

Avian Proteins*

Base Sequence

Chickens

Cloning, Molecular

DNA/genetics

Exons

Female

Humans

Membrane Proteins/genetics*

Molecular Sequence Data

Muscle Proteins/genetics*

Polymerase Chain Reaction

RNA Splicing

RNA, Messenger/genetics

Restriction Mapping

Species Specificity

Swine

Transcription, Genetic

Uterus/metabolism

Vinculin/genetics*