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Research Database PMU-SQQUID

Complex protein composition of isolated focal adhesions: a two-dimensional gel and database analysis.
Niederreiter, M; Gimona, M; Streichsbier, F; Celis, JE; Small, JV;
Electrophoresis. 1994; 15(3-4): 511-519.
Originalarbeiten (Zeitschrift)

PMU-Authors

Gimona Mario

Abstract

Current ideas about the composition of the focal adhesion complexes in cultured cells are based mainly on indirect immunocytochemical data. We here report a two-dimensional (2-D) gel electrophoresis analysis of the focal adhesion associated-structures that remain in the growth substrate after removal of cells by mechanical shearing. Many proteins additional to the known adhesion proteins, and in higher abundance, could be identified. Using selective extraction procedures, employing detergent or gelsolin, these could be classified as either membrane-associated, actin-associated or both. Cross correlation of these polypeptode patterns with a 2-D gel database allowed identification of some proteins, not previously considered as resident of focal adhesions. The data point to a more complex make up of focal adhesions than formerly supposed.


Useful keywords (using NLM MeSH Indexing)

3T3 Cells

Actins/analysis

Animals

Autoradiography/methods

Cell Adhesion*

Cell Line

Cell Line, Transformed

Chick Embryo

Databases, Factual*

Electrophoresis, Gel, Two-Dimensional/methods*

Fluorescent Antibody Technique

Humans

Immunohistochemistry

Iodine Radioisotopes

Mice

Models, Biological

Protein Biosynthesis

Proteins/analysis*

Proteins/isolation*

purification

Vinculin/analysis