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Research Database PMU-SQQUID

CLAMP, a novel microtubule-associated protein with EB-type calponin homology.
Dougherty, GW; Adler, HJ; Rzadzinska, A; Gimona, M; Tomita, Y; Lattig, MC; Merritt, RC; Kachar, B;
Cell Motil Cytoskeleton. 2005; 62(3): 141-156.
Originalarbeiten (Zeitschrift)

PMU-Authors

Gimona Mario

Abstract

Microtubules (MTs) are polymers of a and P tubulin dimers that mediate many cellular functions, including the establishment and maintenance of cell shape. The dynamic properties of MTs may be influenced by tubulin isotype, posttranslational modifications of tubulin, and interaction with microtubule-associated proteins (MAPs). End-binding (EB) family proteins affect MT dynamics by stabilizing MTs, and are the only MAPs reported that bind MTs via a calponin-homology (CH) domain (J Biol Chem 278 (2003) 49721-49731; J Cell Biol 149 (2000) 761766). Here, we describe a novel 27 kDa protein identified from an inner ear organ of Corti library. Structural homology modeling demonstrates a CH domain in this protein similar to EB proteins. Northern and Western blottings confirmed expression of this gene in other tissues, including brain, lung, and testis. In the organ of Corti, this protein localized throughout distinctively large and well-ordered NIT bundles that support the elongated body of mechanically stiff pillar cells of the auditory sensory epithelium. When ectopically expressed in Cos-7 cells, this protein localized along cytoplasmic MTs, promoted NIT bundling, and efficiently stabilized MTs against depolymerization in response to high concentration of nocodazole and cold temperature. We propose that this protein, designated CLAMP, is a novel MAP and represents a new member of the CH domain protein family.


Useful keywords (using NLM MeSH Indexing)

Amino Acid Sequence

Animals

Blotting, Northern

COS Cells

Calcium-Binding Proteins/chemistry

Calcium-Binding Proteins/genetics*

Calcium-Binding Proteins/metabolism

Cercopithecus aethiops

Cloning, Molecular

Conserved Sequence

Cytoskeletal Proteins/chemistry

Cytoskeletal Proteins/genetics

Cytoskeletal Proteins/metabolism

Gene Expression Profiling

Humans

Mice

Microfilament Proteins

Microscopy, Electron, Scanning

Microtubule-Associated Proteins/chemistry

Microtubule-Associated Proteins/genetics*

Microtubule-Associated Proteins/metabolism

Microtubules/metabolism*

Models, Molecular

Molecular Sequence Data

Organ of Corti/metabolism

Polymerase Chain Reaction

Rats

Sequence Homology, Amino Acid

Tubulin/genetics

Tubulin/metabolism


Find related publications in this database (Keywords)

microtubule-associated protein
cytoskeleton
pillar cells
tubulin