' '
Deutsch | English    

Research Database PMU-SQQUID

Zyxin is not colocalized with vasodilator-stimulated phosphoprotein (VASP) at lamellipodial tips and exhibits different dynamics to vinculin, paxillin, and VASP in focal adhesions.
Rottner, K; Krause, M; Gimona, M; Small, JV; Wehland, J;
Mol Biol Cell. 2001; 12(10): 310-313.
Originalarbeiten (Zeitschrift)

PMU-Authors

Gimona Mario

Abstract

Actin polymerization is accompanied by the formation of protein complexes that link extracellular signals to sites of actin assembly such as membrane ruffles and focal adhesions. One candidate recently implicated in these processes is the LIM domain protein zyxin, which can bind both Ena/vasodilator-stimulated phosphoprotein (VASP) proteins and the actin filament cross-linking protein a-actinin. To characterize the localization and dynamics of zyxin in detail, we generated both monoclonal antibodies and a green fluorescent protein (GFP)-fusion construct. The antibodies colocalized with ectopically expressed GFP-VASP at focal adhesions and along stress fibers, but failed to label lamellipodial and filopodial tips, which also recruit Ena/VASP proteins. Likewise, neither microinjected, fluorescently labeled zyxin antibodies nor ectopically expressed GFP-zyxin were recruited to these latter sites in live cells, whereas both probes incorporated into focal adhesions and stress fibers. Comparing the dynamics of zyxin with that of the focal adhesion protein vinculin revealed that both proteins incorporated simultaneously into newly formed adhesions. However, during spontaneous or induced focal adhesion disassembly, zyxin delocalization preceded that of either vinculin or paxiliin. Together, these data identify zyxin as an early target for signals leading to adhesion disassembly, but exclude its role in recruiting Ena/VASP proteins to the tips of lamellipodia and filopodia.


Useful keywords (using NLM MeSH Indexing)

Actinin/metabolism

Animals

Antibodies, Monoclonal/chemistry

Cell Adhesion Molecules/metabolism*

Cytoskeletal Proteins/metabolism

Fibroblasts

Focal Adhesions/metabolism*

Glycoproteins

HeLa Cells

Humans

Metalloproteins/chemistry

Metalloproteins/metabolism*

Mice

Microfilament Proteins

Paxillin

Phosphoproteins/metabolism*

Pseudopodia/metabolism*

Rats

Vinculin/metabolism*

Zyxin