' '
Deutsch | English    

Research Database PMU-SQQUID

Smooth muscle specific expression of calponin.
Gimona, M; Herzog, M; Vandekerckhove, J; Small, JV;
FEBS Lett. 1990; 274(1-2): 159-162.
Originalarbeiten (Zeitschrift)

PMU-Authors

Gimona Mario

Abstract

Calponin is an actin-, calmodulin-, and tropomyosin-binding protein that has been isolated from smooth muscle tissue. Using a monoclonal antibody specific for avian calponin, we demonstrate a differentiation-linked increase in calponin expression in embryonic chick gizzard. Cultivation of gizzard smooth muscle cells in vitro resulted in a down-regulation of calponin expression after the first 48 h that was paralleled by a loss of synthesis of metavinculin and the high molecular weight isoform of caldesmon. In early cultures of smooth muscle cells calponin was localised in the actin-containing stress fibres but labelling was restricted to the central parts of the actin cytoskeleton. Calponin expression is suggested as a potentially useful index of smooth muscle differentiation.


Useful keywords (using NLM MeSH Indexing)

Animals

Antibodies, Monoclonal/diagnostic use

Calcium-Binding Proteins/analysis*

Embryo, Nonmammalian

Fluorescent Antibody Technique

Gizzard/metabolism

Immunoblotting

Microfilament Proteins

Molecular Weight

Muscle Proteins/analysis*

Muscle, Smooth/metabolism*

Turkeys