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Research Database PMU-SQQUID

Calponin. Developmental isoforms and a low molecular weight variant.
Draeger, A; Gimona, M; Stuckert, A; Celis, JE; Small, JV;
FEBS Lett. 1991; 291(1): 24-28.
Originalarbeiten (Zeitschrift)

PMU-Authors

Gimona Mario

Abstract

Two-dimensional gel analysis of basic proteins in developing human smooth muscle identifies calponin as a prominent marker of the differentiated phenotype. Adult tissue (human and mouse) typically expresses up to four calponin isoforms, three of which appear sequentially during fetal development: adult myometrial cells express the same three isoforms in primary culture in vitro and these are down-regulated, in reverse order, during the subsequent modulation of phenotype. Monospecific, polyclonal antibodies against calponin identify a lower molecular weight variant of calponin (L-calponin) that is strongly and specifically expressed in adult smooth muscles of the human urogenital tract. L-calponin is down-regulated in benign smooth muscle derived tumors (leiomyoma) and is not expressed in primary cultures of normal uterine tissue.


Useful keywords (using NLM MeSH Indexing)

Animals

Calcium-Binding Proteins/chemistry

Calcium-Binding Proteins/metabolism*

Cell Differentiation

Culture Techniques

Electrophoresis, Gel, Two-Dimensional

Embryonic and Fetal Development

Female

Humans

Mice

Mice, Inbred BALB C

Microfilament Proteins

Molecular Weight

Muscle Development

Muscle Proteins/chemistry

Muscle Proteins/metabolism*

Muscle, Smooth/cytology

Muscle, Smooth/growth*

development

Muscle, Smooth/metabolism*

Phenotype

Pregnancy


Find related publications in this database (Keywords)

SMOOTH MUSCLE
PROTEIN ISOFORMS
DEVELOPMENT
CALPONIN