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Research Database PMU-SQQUID

Variable and constant regions in the C-terminus of vinculin and metavinculin. Cloning and expression of fragments in E. coli.
Strasser, P; Gimona, M; Herzog, M; Geiger, B; Small, JV;
FEBS Lett. 1993; 317(3): 189-194.
Originalarbeiten (Zeitschrift)

PMU-Authors

Gimona Mario
Strasser Peter

Abstract

Metavinculin differs from vinculin in having an additional insert of 68 to 79 amino acids in length in the C-terminal half of the molecule. Cross-species comparison of metavinculin sequences from pig, man, chicken and frog reveals a division of the insert into two parts: the first variable and the second highly conserved. The longest insert, 79 amino acids, was found in Xenopus laevis. Three different C-terminal constructs of vinculin and metavinculin over-expressed in E. coli could be purified by column chromatography. Two-dimensional gel electrophoresis and peptide analysis revealed pI values between 8.35 and 10.25 for the recombinant proteins. Biochemical and structural features of the metavinculin-specific sequence and the conserved vinculin/metavinculin carboxy-terminus are discussed.


Useful keywords (using NLM MeSH Indexing)

Amino Acid Sequence

Animals

Avian Proteins*

Cloning, Molecular

Conserved Sequence

Escherichia coli/metabolism

Humans

Molecular Sequence Data

Muscle Proteins/biosynthesis

Muscle Proteins/chemistry*

Recombinant Proteins/biosynthesis

Recombinant Proteins/chemistry

Sequence Homology, Amino Acid

Species Specificity

Vinculin/biosynthesis

Vinculin/chemistry*


Find related publications in this database (Keywords)

VINCULIN
METAVINCULIN
C-TERMINUS
SEQUENCE
XENOPUS
ESCHERICHIA-COLI EXPRESSION