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Research Database PMU-SQQUID

Beta-actin specific monoclonal antibody.
Gimona, M; Vandekerckhove, J; Goethals, M; Herzog, M; Lando, Z; Small, JV;
Cell Motil Cytoskeleton. 1994; 27(2): 108-116.
Originalarbeiten (Zeitschrift)

PMU-Authors

Gimona Mario

Abstract

Using a synthetic peptide mimicking the NH2-terminus of beta-actin we have raised a monoclonal antibody specific for this cytoplasmic actin isoform. Specificity of the antibody was demonstrated by its labelling of the actin polypeptide only in tissues containing the beta isoform, by its exclusive recognition of the synthetic beta-actin peptide amongst those mimicking all six vertebrate isoactins, and by its selective recognition of the beta-actin spot in two-dimensional electrophoresis gels of smooth muscle extracts. The antibody bound to actin filaments in both living and fixed fibroblasts where it labelled the stress fiber bundles and, more predominantly, the peripheral actin rich lamellipodia. The characteristics of the antibody indicate that it should serve as a useful tool for studying isoactin distribution and function. (C) 1994 Wiley-Liss, Inc.


Useful keywords (using NLM MeSH Indexing)

Actins/analysis*

Actins/chemical synthesis

Actins/immunology*

Amino Acid Sequence

Animals

Antibodies, Monoclonal*

Antibody Specificity

Cell Line

Chick Embryo

Chickens

Cytoskeleton/ultrastructure

Fibroblasts/chemistry

Gizzard/chemistry

Mice

Molecular Sequence Data

Muscles/chemistry

Myocardium/chemistry

Peptide Fragments/chemical synthesis

Peptide Fragments/immunology

Rats


Find related publications in this database (Keywords)

SMOOTH MUSCLE
FIBROBLASTS
LAMELLIPODIA