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Research Database PMU-SQQUID

Functional interaction in establishment of ribosomal integrity between small subunit protein rpS6 and translational regulator rpL10/Grc5p.
Pachler, K; Karl, T; Kolmann, K; Mehlmer, N; Eder, M; Loeffler, M; Oender, K; Hochleitner, EO; Lottspeich, F; Bresgen, N; Richter, K; Breitenbach, M; Koller, L;
FEMS Yeast Res. 2004; 5(3):271-280
Full papers/articles (Journal)

PMU-Authors

├ľnder Kamil
Pachler Karin

Abstract

Functional ribosomes synthesize proteins in all living cells and are composed of two labile associated subunits, which are made of rRNA and ribosomal proteins. The rRNA of the small 40S subunit (SSU) of the functional eukaryotic 80S ribosome decodes the mRNA molecule and the large 60S subunit (LSU) rRNA catalyzes protein synthesis. Recent fine structure determinations of the ribosome renewed interest in the role of ribosomal proteins in modulation of the core ribosomal functions. RpL10/Grc5p is a component of the LSU and is a multifunctional translational regulator, operating in 60S subunit biogenesis, 60S subunit export and 60S subunit joining with the 40S subunit. Here, we report that rpL10/Grc5p functionally interacts with the nuclear export factor Nmd3p in modulation of the cellular polysome complement and with the small subunit protein rpS6 in subunit joining and differential protein expression.


Useful keywords (using NLM MeSH Indexing)

Base Sequence

DNA, Fungal/chemistry

DNA, Fungal/genetics

Electrophoresis, Gel, Two-Dimensional

Fungal Proteins/genetics

Fungal Proteins/metabolism

Gene Expression Regulation, Fungal

Molecular Sequence Data

Polymerase Chain Reaction

Ribosomal Protein S6/genetics

Ribosomal Protein S6/physiology*

Ribosomal Proteins/genetics

Ribosomal Proteins/physiology*

Ribosomes/genetics

Ribosomes/physiology*

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Yeasts/genetics

Yeasts/physiology*


Find related publications in this database (Keywords)

translational control
ribosomal protein
rpL10/Grc5p
rpS6
differential protein expression