The effect of follicle-stimulating hormone (FSH) on the electrical properties of TM4-Sertoli cells was investigated. Addition of 5 IU/ml FSH caused a dose-dependent and reversible depolarization of the resting membrane potential by +15.3 +/- 1.0 mV accompanied by a decrease of the input resistance. The depolarization was completely abolished in chloride-free solutions. The reversal potential of the effect was close to the calculated reversal potential for chloride. We conclude that FSH activates a chloride conductance in cultured TM4 Sertoli cells.
Useful keywords (using NLM MeSH Indexing)
Follicle Stimulating Hormone/pharmacology*
Membrane Potentials/drug effects
Mice, Inbred BALB C
Sertoli Cells/drug effects*