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Research Database PMU-SQQUID

Blockade of swelling-induced chloride channels by phenol derivatives.
Gschwentner, M; Jungwirth, A; Hofer, S; Wöll, E; Ritter, M; Susanna, A; Schmarda, A; Reibnegger, G; Pinggera, GM; Leitinger, M; Frick, J; Deetjen, P; Paulmichl, M;
Br J Pharmacol. 1996; 118(1): 41-48.
Originalarbeiten (Zeitschrift)

PMU-Authors

Paulmichl Markus
Ritter Markus

Abstract

1. In NIH3T3 fibroblasts, the chloride channel involved in regulatory volume decrease (RVD) was identified as ICln, a protein isolated from a cDNA library derived from Madin Darby canine Kidney (MDCK) cells. ICln expressed in Xenopus laevis oocytes gives rise to an outwardly rectifying chloride current, sensitive to the extracellular addition of nucleotides and the known chloride channel blockers, DIDS (4,4"-diisothiocyanatostilbene-2,2"-disulphonic acid) and NPPB (5-nitro-2-(3-phenylpropylamino)-benzoic acid). We set out to study whether substances structurally similar to NPPB are able to interfere with RVD. 2. RVD in NIH3T3 fibroblasts and MDCK cells is temperature-dependent. 3. RVD, the swelling-dependent chloride current and the depolarization seen after reducing extracellular osmolarity can be blocked by gossypol and NDGA (nordihydroguaiaretic acid), both structurally related to NPPB. 4. The cyclic AMP-dependent chloride current elicited in CaCo cells is less sensitive to the two substances tested while the calcium-activated chloride current in fibroblasts is insensitive. 5. The binding site for the two phenol derivatives onto ICln seems to be distinct but closely related to the nucleotide binding site identified as G x G x G, a glycine repeat located at the predicted outer mouth of the ICln channel protein.


Useful keywords (using NLM MeSH Indexing)

3T3 Cells/cytology

3T3 Cells/drug effects

Animals

Binding Sites

Caco-2 Cells/cytology

Caco-2 Cells/drug effects

Calcium/physiology

Cell Size/drug effects

Cell Size/physiology

Chloride Channels/antagonists*

inhibitors*

Chloride Channels/physiology*

Chlorides/metabolism

Chlorides/physiology

Cyclic AMP/physiology

Dogs

Gossypol/pharmacology

Humans

Kidney/cytology

Kidney/drug effects

Membrane Potentials/drug effects

Mice

Nordihydroguaiaretic Acid/pharmacology

Phenols/pharmacology*

Thymine Nucleotides/pharmacology

Xenopus laevis


Find related publications in this database (Keywords)

fibroblasts
MDCK
volume regulation
gossypol
NDGA
patch clamp
chloride currents
I-Cln