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Research Database PMU-SQQUID

A functional corona around extracellular vesicles enhances angiogenesis, skin regeneration and immunomodulation.
Wolf, M; Poupardin, RW; Ebner-Peking, P; Andrade, AC; Blöchl, C; Obermayer, A; Gomes, FG; Vari, B; Maeding, N; Eminger, E; Binder, HM; Raninger, AM; Hochmann, S; Brachtl, G; Spittler, A; Heuser, T; Ofir, R; Huber, CG; Aberman, Z; Schallmoser, K; Volk, HD; Strunk, D;
J Extracell Vesicles. 2022; 11(4): e12207
Full papers/articles (Journal)


Binder Heide-Marie
Brachtl Gabriele
Cronemberger Andrade André
Ebner Patricia
Gueths Gomes Fausto
Hochmann Sarah
Maeding Nicole
Raninger Anna
Schallmoser Katharina
Strunk Dirk
Vári Balázs
Wolf Martin


Nanoparticles can acquire a plasma protein corona defining their biological identity. Corona functions were previously considered for cell-derived extracellular vesicles (EVs). Here we demonstrate that nano-sized EVs from therapy-grade human placental-expanded (PLX) stromal cells are surrounded by an imageable and functional protein corona when enriched with permissive technology. Scalable EV separation from cell-secreted soluble factors via tangential flow-filtration (TFF) and subtractive tandem mass-tag (TMT) proteomics revealed significant enrichment of predominantly immunomodulatory and proangiogenic proteins. Western blot, calcein-based flow cytometry, super-resolution and electron microscopy verified EV identity. PLX-EVs partly protected corona proteins from protease digestion. EVs significantly ameliorated human skin regeneration and angiogenesis in vivo, induced differential signalling in immune cells, and dose-dependently inhibited T cell proliferation in vitro. Corona removal by size-exclusion or ultracentrifugation abrogated angiogenesis. Re-establishing an artificial corona by cloaking EVs with fluorescent albumin as a model protein or defined proangiogenic factors was depicted by super-resolution microscopy, electron microscopy and zeta-potential shift, and served as a proof-of-concept. Understanding EV corona formation will improve rational EV-inspired nano-therapy design.

Useful keywords (using NLM MeSH Indexing)

Extracellular Vesicles*/metabolism






Protein Corona*/metabolism


Find related publications in this database (Keywords)

EV corona
EV function
extracelular vesicle
placenta derived stromal cells
tangential flow filtration