Research Database PMU-SQQUID

A Swelling-Activated Chloride Current in Microglial Cells is Suppressed by Epac and Facilitated by PKA - Impact on Phagocytosis.
Kittl, M; Jakab, M; Steininger, TS; Ritter, M; Kerschbaum, HH;
Cell Physiol Biochem. 2019; 52(5): 951-969.
Full papers/articles (Journal)

Abstract/Keywords PubMed Full Text

PMU-Authors

Jakab Martin
Kittl Michael
Ritter Markus

Abstract

BACKGROUND/AIMS
Volume-regulated anion channels (VRACs) are of particular importance in regulating the cell volume (CV) and give rise to the swelling-activated Cl- current (I
Experiments were performed in murine microglial BV-2 and primary mouse microglial cells. Patch clamp experiments were performed in BV-2 cells using the amphotericin-perforated method to minimize cytosolic disturbances. Phagocytosis was quantified by scanning electron microscopy.
Following activation of I
The activation of β-adrenergic receptors in microglial cells triggers a cAMP-Epac-dependent and a cAMP-PKA-dependent cascade which affects phagocytosis via modulation of the swelling-activated Cl- current I