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Research Database PMU-SQQUID

Mesenchymal stem cells promote oligodendroglial differentiation in hippocampal slice cultures.
Rivera, FJ; Siebzehnrubl, FA; Kandasamy, M; Couillard-Despres, S; Caioni, M; Poehler, AM; Berninger, B; Sandner, B; Bogdahn, U; Goetz, M; Bluemcke, I; Weidner, N; Aigner, L;
Cell Physiol Biochem. 2009; 24(3-4):317-324
Originalarbeiten (Zeitschrift)

PMU-Authors

Aigner Ludwig
Couillard-Després Sébastien
Rivera Gomez-Barris Francisco J.

Abstract

We have previously shown that soluble factors derived from mesenchymal stem cells (MSCs) induce oligodendrogenic fate and differentiation in adult rat neural progenitors (NPCs) in vitro. Here, we investigated if this pro-oligodendrogenic effect is maintained after cells have been transplanted onto rat hippocampal slice cultures, a CNS-organotypic environment. We first tested whether NPCs, that were pre-differentiated in vitro by MSC-derived conditioned medium, would generate oligodendrocytes after transplantation. This approach resulted in the loss of grafted NPCs, suggesting that oligodendroglial pre-differentiated cells could not integrate in the tissue and therefore did not survive grafting. However, when NPCs together with MSCs were transplanted in situ into hippocampal slice cultures, the grafted NPCs survived and the majority of them differentiated into oligodendrocytes. In contrast to the prevalent oligodendroglial differentiation in case of the NPC/MSC co-transplantation, naive NPCs transplanted in the absence of MSCs differentiated predominantly into astrocytes. In summary, the pro-oligodendrogenic activity of MSCs was maintained only after co-transplantation into hippocampal slice cultures. Therefore, in the otherwise astrogenic milieu, MSCs established an oligodendrogenic niche for transplanted NPCs, and thus, co-transplantation of MSCs with NPCs might provide an attractive approach to re-myelinate the various regions of the diseased CNS. Copyright (C) 2009 S. Karger AG, Basel


Useful keywords (using NLM MeSH Indexing)

Animals

Antimetabolites/metabolism

Bromodeoxyuridine/metabolism

Cell Differentiation/physiology*

Female

Femur/cytology

Hippocampus/metabolism*

Mesenchymal Stromal Cells/cytology*

Mesenchymal Stromal Cells/metabolism*

Oligodendroglia/metabolism*

Organ Culture Techniques

Rats

Rats, Inbred F344

Rats, Wistar

Tibia/cytology


Find related publications in this database (Keywords)

Mesenchymal stem cells
Neural progenitors
Oligodendrocyte differentiation
Hippocampal slice culture
Multiple Sclerosis