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Research Database PMU-SQQUID

Thiolated polyacrylic acid-modified iron oxide nanoparticles for in vitro labeling and MRI of stem cells.
Vetter, A; Reinisch, A; Strunk, D; Kremser, C; Hahn, HW; Huck, CW; Ostermann, T; Leithner, K; Bernkop-Schnurch, A
J DRUG TARGET. 2011; 19(7): 56-72.
Originalarbeiten (Zeitschrift)

PMU-Authors

Strunk Dirk

Abstract

Purpose: The purpose of this study was to develop and characterize new surface-modified iron oxide nanoparticles demonstrating the efficiency to be internalized by human endothelial progenitor cells (EPCs) from umbilical cord blood. Methods: Iron oxide nanoparticles were coated with polyacrylic acid-cysteine (PAA-Cys) by either in situ precipitation or postsynthesis. The nanoparticles were characterized by X-ray powder diffraction. EPCs were labeled with PAA-Cys-modified iron oxide nanoparticles or with uncoated nanoparticles. The relaxivity of uncoated and coated iron oxide nanoparticles as well as EPCs labeled with PAA-Cys-modified iron oxide were determined. Results: Addition of PAA-Cys increased the particle size from 10.4 to 144 and 197 nm, respectively. The X-ray powder diffraction pattern revealed that the particles consist of Fe(3)O(4) with a spinal structure. Postsynthesis coated particles showed a cellular uptake of 85% and 15.26 pg iron/cell. For both types of particles the relaxivity ratio was at least 2-fold higher than that of the gold standard Resovist . Conclusion: The PAA-Cys coated iron oxide nanoparticles are a promising tool for labeling living cells such as stem cells for diagnostic and therapeutic application in cell-based therapies due to their high relaxivities and their easy uptake by cells.


Useful keywords (using NLM MeSH Indexing)

Acrylic Resins/chemistry*

Cells, Cultured

Ferric Compounds/chemistry*

Humans

Magnetic Resonance Imaging*

Metal Nanoparticles*

Powder Diffraction

Spectroscopy, Fourier Transform Infrared

Sulfhydryl Compounds/chemistry*


Find related publications in this database (Keywords)

Stem cells
nanoparticles
iron oxide
thiomers
relaxivity