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Forschungsdatenbank PMU-SQQUID

Liquid-based cytology as a tool for the performance of uCyt+ and Urovysion Multicolour-FISH in the detection of urothelial carcinoma.
Mian, C; Lodde, M; Comploj, E; Negri, G; Egarter-Vigl, E; Lusuardi, L; Palermo, S; Marberger, M; Pycha, A;
Cytopathology. 2003; 14(6): 338-342.
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Lusuardi Lukas

Abstract

The aim of the study was to assess the value of liquid-based urinary cytology as a tool to perform uCyt+ and Multicolour-FISH in patients under follow-up after urothelial cancer. Therefore, standard cytology was compared to liquid-based cytology with the addition of the uCyt+ test, which traces the three monoclonal antibodies M344, LDQ10 and 19A211 in exfoliated urothelial cells; and Multicolour-FISH (including centromere-specific probes for chromosomes 3, 7, 17 and a locus-specific probe for 9p21/p16) performed on thin-layer specimens. UCyt+ showed an overall sensitivity of 86.2% and cytology of 45.0%. Overall sensitivity of both the tests combined was 90%. Sensitivity of Multicolour-FISH was 96.4%. All conventional cytology diagnoses were confirmed by liquid-based cytology. Liquid-based cytology is a valid tool for the performance of adjunctive analyses, such as uCyt+ and Multicolour-FISH, on residual cellular material.


Useful keywords (using NLM MeSH Indexing)

Adult

Aged

Aged, 80 and over

Carcinoma, Transitional Cell/diagnosis*

Carcinoma, Transitional Cell/urine

Cytodiagnosis/instrumentation

Cytodiagnosis/methods*

Humans

Immunohistochemistry/methods

Immunohistochemistry/standards

In Situ Hybridization, Fluorescence*

Middle Aged

Predictive Value of Tests

Prospective Studies

Sensitivity and Specificity

Tumor Markers, Biological/urine

Urinary Bladder Neoplasms/diagnosis*

Urinary Bladder Neoplasms/urine

Urothelium/pathology*


Find related publications in this database (Keywords)

urothelial cancer
uCyt
Multicolour-FISH
liquid-based cytology