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Forschungsdatenbank PMU-SQQUID

Correlation of clinical outcome with p53 and p21 status in patients with advanced transitional-cell carcinoma treated with paclitaxel and carboplatin.
Flamm, M; Brodowicz, T; Haitel, A; Susani, M; Tomek, S; Köstler, W; Pycha, A; Kratzik, C; Marberger, M; Zielinski, CC;
Anticancer Res. 2002; 22(2B):1295-1300
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Flamm Maria

Abstract

Fgf-8 encodes a secreted signaling molecule mediating key roles in embryonic patterning. This study analyzes the expression pattern, regulation, and function of this growth factor in the paraxial mesoderm of the avian embryo. In the mature somite, expression of Fgf-8 is restricted to a subpopulation of myotome cells, comprising most, but not all, epaxial and hypaxial muscle precursors. Following ablation of the notochord and floor plate, Fgf-8 expression is not activated in the somites, in either the epaxial or the hypaxial domain, while ablation of the dorsal neural tube does not affect Fgf-8 expression in paraxial mesoderm. Contrary to the view that hypaxial muscle precursors are independent of regulatory influences from axial structures, these findings provide the first evidence for a regulatory influence of ventral, but not dorsal axial structures on the hypaxial muscle domain. Sonic hedgehog can substitute for the ventral neural tube and notochord in the initiation of Fgf-8 expression in the myotome. It is also shown that Fgf-8 protein leads to an increase in sclerotomal cell proliferation and enhances rib cartilage development in mature somites, whereas inhibition of Fgf signaling by SU 5402 causes deletions in developing ribs. These observations demonstrate: (1) a regulatory influence of the ventral axial organs on the hypaxial muscle compartment; (2) regulation of epaxial and hypaxial expression of Fgf-8 by Sonic hedgehog; and (3) independent regulation of Fgf-8 and MyoD in the hypaxial myotome by ventral axial organs. It is postulated that the notochord and ventral neural tube influence hypaxial expression of Fgf-8 in the myotome and that, in turn, Fgf-8 has a functional role in rib formation.


Useful keywords (using NLM MeSH Indexing)

Aged

Antineoplastic Combined Chemotherapy Protocols/therapeutic use*

Carboplatin/administration*

dosage

Carcinoma, Transitional Cell/drug therapy*

Carcinoma, Transitional Cell/metabolism*

Carcinoma, Transitional Cell/secondary

Cell Nucleus/metabolism

Cyclin-Dependent Kinase Inhibitor p21

Cyclins/biosynthesis*

Disease-Free Survival

Female

Humans

Immunohistochemistry

Male

Middle Aged

Paclitaxel/administration*

dosage

Tumor Suppressor Protein p53/biosynthesis*

Urologic Neoplasms/drug therapy*

Urologic Neoplasms/metabolism*

Urologic Neoplasms/pathology


Find related publications in this database (Keywords)

paclitaxel
carboplatin
p21
p53
transitional-cell carcinoma