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Forschungsdatenbank PMU-SQQUID

Immunomodulative efficacy of bone marrow-derived mesenchymal stem cells cultured in human platelet lysate.
Flemming, A; Schallmoser, K; Strunk, D; Stolk, M; Volk, HD; Seifert, M;
J Clin Immunol. 2011; 31(6): 1143-1156.
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Schallmoser Katharina
Strunk Dirk

Abstract

Human mesenchymal stem cells (hMSCs) are considered to be a promising tool for novel cell-based therapies. Clinical applications in solid organ transplantation were hampered by the dependence on animal serum for hMSCs clinical scale expansion until substitution with human platelet lysate (HPL) became a promising alternative. Therefore we focused on a direct comparison of immunomodulatory properties of hMSCs cultured in HPL or fetal calf serum (FCS). Phenotypic characterization, detection of cytokine secretion and effects on alloantigen- and mitogen-induced lymphocyte proliferation as well as degranulation of cytomegalovirus-specific cytotoxic T cells were applied in potency assays. We demonstrated that HPL-cultured MSCs have comparable immunomodulatory capacities to their FCS-cultured counterparts. The observed immunomodulatory properties include a beneficial inhibitory effect on immune cell proliferation and an unaffected viral T cell immunity. Thus, culturing hMSCs in HPL generates an efficient and safe expansion combined with intriguing immunomodulatory properties making these cells an attractive cell therapeutic tool.


Useful keywords (using NLM MeSH Indexing)

Animals

Antigens, Viral/immunology

Blood Platelets/metabolism*

Bone Marrow/pathology

Cattle

Cell Extracts/immunology*

Cell Proliferation

Cells, Cultured

Culture Media/metabolism

Cytomegalovirus/immunology*

Cytotoxicity, Immunologic

Humans

Immunomodulation*

Isoantigens/immunology

Mesenchymal Stem Cell Transplantation*

Mesenchymal Stromal Cells/immunology

Mesenchymal Stromal Cells/metabolism*

Mesenchymal Stromal Cells/pathology

Serum/immunology*

Serum/metabolism

T-Lymphocytes, Cytotoxic/immunology

T-Lymphocytes, Cytotoxic/metabolism*

T-Lymphocytes, Cytotoxic/pathology


Find related publications in this database (Keywords)

Immunomodulation
mesenchymal stem cell
human platelet lysate
cell therapy