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Forschungsdatenbank PMU-SQQUID

Nuclear Zonula occludens-2 alters gene expression and junctional stability in epithelial and endothelial cells.
Traweger, A; Lehner, C; Farkas, A; Krizbai, IA; Tempfer, H; Klement, E; Guenther, B; Bauer, HC; Bauer, H;
Differentiation. 2008; 76(1): 99-106.
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Bauer Hans Christian
Lehner Christine
Tempfer Herbert
Traweger Andreas

Abstract


Zonula occludens proteins (ZOPs) are essential scaffold proteins involved in the organization of epithelial and endothelial intercellular junctions. Based on their molecular domain architecture, they are members of the large family of membrane-associated guanylate kinase-like (MAGUK) proteins. As all other MAGUKs, ZOPs contain a core of several PDZ, an src homology-3, and a guanylate kinase-like domain, indicating that these proteins may serve both structural and signaling functions. In addition, ZOPs exhibit some unique motifs not shared by other MAGUKs, i.e., several nuclear localization (NLS) and nuclear export signals (NES), allowing these proteins to shuttle between the cytoplasm and the nucleus. However, the stimuli leading to the nuclear accumulation of ZOPs and the resulting physiological consequences remain poorly defined. We have previously reported the direct binding of nuclear ZO-2 to scaffold attachment factor B, a heterogeneous nuclear ribonucleoprotein involved in chromatin organization and the transcriptional control of eukaryotic genes. We now report that the nuclear accumulation of ZO-2 leads to an increase in the expression of the M2 type of pyruvate kinase (M2-PK) in epithelial and endothelial cells. Further, the proliferative activity was increased, while the intercellular junctional stability of Madin-Darby canine kidney cells was reduced. Our data provide evidence to suggest that ZO-2 exerts a junction-unrelated function that further supports the notion of a general "dual" role of junctional MAGUKs, being an indispensable structural component at cell-cell junctions and a nuclear factor influencing gene expression and cell behavior.


Useful keywords (using NLM MeSH Indexing)

Animals

Calcium Signaling

Cell Line

Cell Nucleus/genetics

Cell Nucleus/metabolism

Cell Proliferation

Dogs

Endothelial Cells/physiology*

Epithelial Cells/physiology*

Gene Expression Regulation/physiology

Intercellular Junctions/metabolism

Membrane Proteins/genetics*

Membrane Proteins/metabolism*

Nuclear Proteins/genetics

Nuclear Proteins/metabolism*

Pyruvate Kinase/biosynthesis

Transfection

Zonula Occludens-2 Protein


Find related publications in this database (Keywords)

ZO-2
nuclear localization
M2 pyruvate kinase