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Forschungsdatenbank PMU-SQQUID

Molecular characterisation of transport mechanisms at the developing mouse blood-CSF interface: a transcriptome approach.
Liddelow, SA; Temple, S; Møllgård, K; Gehwolf, R; Wagner, A; Bauer, H; Bauer, HC; Phoenix, TN; Dziegielewska, KM; Saunders, NR;
PLoS One. 2012; 7(3): e33554
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Bauer Hans Christian
Gehwolf Renate
Wagner Andrea

Abstract

Exchange mechanisms across the blood-cerebrospinal fluid (CSF) barrier in the choroid plexuses within the cerebral ventricles control access of molecules to the central nervous system, especially in early development when the brain is poorly vascularised. However, little is known about their molecular or developmental characteristics. We examined the transcriptome of lateral ventricular choroid plexus in embryonic day 15 (E15) and adult mice. Numerous genes identified in the adult were expressed at similar levels at E15, indicating substantial plexus maturity early in development. Some genes coding for key functions (intercellular/tight junctions, influx/efflux transporters) changed expression during development and their expression patterns are discussed in the context of available physiological/permeability results in the developing brain. Three genes: Secreted protein acidic and rich in cysteine (Sparc), Glycophorin A (Gypa) and C (Gypc), were identified as those whose gene products are candidates to target plasma proteins to choroid plexus cells. These were investigated using quantitative- and single-cell-PCR on plexus epithelial cells that were albumin- or total plasma protein-immunopositive. Results showed a significant degree of concordance between plasma protein/albumin immunoreactivity and expression of the putative transporters. Immunohistochemistry identified SPARC and GYPA in choroid plexus epithelial cells in the embryo with a subcellular distribution that was consistent with transport of albumin from blood to cerebrospinal fluid. In adult plexus this pattern of immunostaining was absent. We propose a model of the cellular mechanism in which SPARC and GYPA, together with identified vesicle-associated membrane proteins (VAMPs) may act as receptors/transporters in developmentally regulated transfer of plasma proteins at the blood-CSF interface.


Useful keywords (using NLM MeSH Indexing)

Amino Acids/metabolism

Animals

Base Sequence

Biological Transport

Blood*

Blotting, Western

Cerebrospinal Fluid*

Choroid Plexus/embryology

DNA Primers

Female

Immunohistochemistry

Mice

Oligonucleotide Array Sequence Analysis

Pregnancy

Protein Binding

Real-Time Polymerase Chain Reaction

Transcriptome*