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Forschungsdatenbank PMU-SQQUID

Doublecortin expression levels in adult brain reflect neurogenesis.
Couillard-Despres, S; Winner, B; Schaubeck, S; Aigner, R; Vroemen, M; Weidner, N; Bogdahn, U; Winkler, J; Kuhn, HG; Aigner, L;
Eur J Neurosci. 2005; 21(1):-14
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Aigner Ludwig
Couillard-Després Sébastien

Abstract


Progress in the field of neurogenesis is currently limited by the lack of tools enabling fast and quantitative analysis of neurogenesis in the adult brain. Doublecortin (DCX) has recently been used as a marker for neurogenesis. However, it was not clear whether DCX could be used to assess modulations occurring in the rate of neurogenesis in the adult mammalian central nervous system following lesioning or stimulatory factors. Using two paradigms increasing neurogenesis levels (physical activity and epileptic seizures), we demonstrate that quantification of DCX-expressing cells allows for an accurate measurement of modulations in the rate of adult neurogenesis. Importantly, we excluded induction of DCX expression during physiological or reactive gliogenesis and excluded also DCX re-expression during regenerative axonal growth. Our data validate DCX as a reliable and specific marker that reflects levels of adult neurogenesis and its modulation. We demonstrate that DCX is a valuable alternative to techniques currently used to measure the levels of neurogenesis. Importantly, in contrast to conventional techniques, analysis of neurogenesis through the detection of DCX does not require in vivo labelling of proliferating cells, thereby opening new avenues for the study of human neurogenesis under normal and pathological conditions.


Useful keywords (using NLM MeSH Indexing)

Animals

Animals, Newborn

Behavior, Animal

Brain/cytology*

Brain/metabolism

Bromodeoxyuridine/metabolism

Cell Count

Cell Differentiation/physiology

Cell Proliferation

Cell Size

Cells, Cultured

Female

GAP-43 Protein/metabolism

Ganglia, Spinal

Gene Expression Regulation/physiology*

Glial Fibrillary Acidic Protein/metabolism

Immunohistochemistry/methods

Indoles/diagnostic use

Laminectomy/methods

Mice

Mice, Inbred C57BL

Microtubule-Associated Proteins/metabolism*

Neurofilament Proteins/metabolism

Neurons/metabolism*

Neuropeptides/metabolism*

Organ Culture Techniques

Phosphopyruvate Hydratase/metabolism

Running

Scopolamine Hydrobromide

Seizures/chemically induced

Seizures/metabolism

Spinal Cord Injuries/metabolism

Stem Cells/metabolism

Time Factors


Find related publications in this database (Keywords)

neural stem cells
neurogenesis indicator
neuronal differentiation
neuronal progenitor