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Forschungsdatenbank PMU-SQQUID

Transient expression of doublecortin during adult neurogenesis.
Brown, JP; Couillard-Després, S; Cooper-Kuhn, CM; Winkler, J; Aigner, L; Kuhn, HG;
J Comp Neurol. 2003; 467(1): 1-10.
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Aigner Ludwig
Couillard-Després Sébastien

Abstract


During development of the central nervous system, expression of the microtubule binding protein doublecortin (DCX) is associated with migration of neuroblasts. In addition to this developmental role, expression of DCX remains high within certain areas of the adult mammalian brain. These areas, mainly the dentate gyrus and the lateral ventricle wall in conjunction with the rostral migratory stream and olfactory bulb, retain the capacity to generate new neurons into adulthood. Adult neurogenesis is typically detected by incorporation of bromodeoxyuridine (BrdU) into dividing cells and colabeling of BrdU-positive cells with markers for mature neurons. To elucidate whether DCX could act as an alternative indicator for adult neurogenesis, we investigated the temporal expression pattern of DCX in neurogenic regions of the adult brain. Analysis of newly generated cells showed that DCX is transiently expressed in proliferating progenitor cells and newly generated neuroblasts. As the newly generated cells began expressing mature neuronal markers, DCX immunoreactivity decreased sharply below the level of detection and remained undetectable thereafter. The transient expression pattern of DCX in neuronal committed progenitor cells/neuroblasts indicates that DCX could be developed into a suitable marker for adult neurogenesis and may provide an alternative to BrdU labeling. This assumption is further supported by our observation that the number of DCX-expressing cells in the dentate gyrus was decreased with age according to the reduction of neurogenesis in the aging dentate gyrus previously reported.


Useful keywords (using NLM MeSH Indexing)

Aging/metabolism*

Animals

Blotting, Western

Bromodeoxyuridine/diagnostic use

Cell Differentiation

Cell Movement

Central Nervous System/growth*

development

Central Nervous System/metabolism*

Dentate Gyrus/metabolism

Electrophoresis, Polyacrylamide Gel

Female

Fluorescent Antibody Technique

Hippocampus/metabolism

Lateral Ventricles/metabolism

Microtubule-Associated Proteins*

Mitosis*

Neurons/metabolism*

Neuropeptides/diagnostic use*

Neuropeptides/metabolism*

Olfactory Bulb/metabolism

Rats

Rats, Wistar

Stem Cells/metabolism

Time Factors


Find related publications in this database (Keywords)

neuronal differentiation
neuronal migration
dentate gyrus
olfactory bulb