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Forschungsdatenbank PMU-SQQUID

Calponin and SM 22 as differentiation markers of smooth muscle: spatiotemporal distribution during avian embryonic development.
Duband, JL; Gimona, M; Scatena, M; Sartore, S; Small, JV;
Differentiation. 1993; 55(1): 1-11.
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Gimona Mario

Abstract


Calponin and SM 22 are two proteins related in sequence that are particularly abundant in smooth muscle cells. Here, the distribution patterns of calponin and SM 22 were compared with that of other smooth muscle contractile and cytoskeletal components in the avian embryo using immunofluorescence microscopy and immunoblotting. Like myosin-light-chain kinase and heavy caldesmon, both calponin and SM 22 were more or less exclusively found in smooth muscle cells, during embryonic development and in the adult. Labelling of other cell types including striated muscle was not observed. In contrast, tropomyosin, smooth muscle alpha-actin, filamin and desmin could also be detected in many other cell types in addition to smooth muscles, at least during part of embryonic life. Calponin and SM 22 appeared almost synchronously during the differentiation of all smooth muscle cell populations, though with a slight time difference in the case of the aorta. The appearance of calponin, SM 22 and heavy caldesmon was generally delayed in relation to desmin, tropomyosin, smooth muscle alpha-actin, myosin-light-chain kinase and filamin and a marked increase in abundance of these proteins was observed in the late embryo and in the adult. From these observations we can conclude that both calponin and SM 22 belong to a group of late differentiation determinants in smooth muscle and may constitute convenient and reliable markers to follow the differentiation of most, if not all, smooth muscle cell populations.


Useful keywords (using NLM MeSH Indexing)

Actins/analysis

Animals

Antibodies, Monoclonal

Antigens, Differentiation/analysis*

Antigens, Differentiation/physiology

Calcium-Binding Proteins/analysis*

Calcium-Binding Proteins/physiology

Calmodulin-Binding Proteins/analysis

Cell Differentiation/drug effects

Cell Differentiation/physiology

Chick Embryo

Contractile Proteins/analysis

Desmin/analysis

Electrophoresis, Polyacrylamide Gel

Embryo, Mammalian/chemistry

Embryo, Mammalian/cytology

Embryo, Nonmammalian

Filamins

Fluorescent Antibody Technique

Immunoblotting

Microfilament Proteins/analysis

Muscle Proteins/analysis*

Muscle Proteins/physiology

Muscle, Smooth, Vascular/chemistry*

Muscle, Smooth, Vascular/cytology

Muscle, Smooth, Vascular/embryology

Myosin-Light-Chain Kinase/analysis

Trypsinogen/analysis