PMU-Autor/inn/en
Önder KamilAbstract
Functional ribosomes synthesize proteins in all living cells and are composed of two labile associated subunits, which are made of rRNA and ribosomal proteins. The rRNA of the small 40S subunit (SSU) of the functional eukaryotic 80S ribosome decodes the mRNA molecule and the large 60S subunit (LSU) rRNA catalyzes protein synthesis. Recent fine structure determinations of the ribosome renewed interest in the role of ribosomal proteins in modulation of the core ribosomal functions. RpL10/Grc5p is a component of the LSU and is a multifunctional translational regulator, operating in 60S subunit biogenesis, 60S subunit export and 60S subunit joining with the 40S subunit. Here, we report that rpL10/Grc5p functionally interacts with the nuclear export factor Nmd3p in modulation of the cellular polysome complement and with the small subunit protein rpS6 in subunit joining and differential protein expression.
Useful keywords (using NLM MeSH Indexing)
Base Sequence
DNA, Fungal/chemistry
DNA, Fungal/genetics
Electrophoresis, Gel, Two-Dimensional
Fungal Proteins/genetics
Fungal Proteins/metabolism
Gene Expression Regulation, Fungal
Molecular Sequence Data
Polymerase Chain Reaction
Ribosomal Protein S6/genetics
Ribosomal Protein S6/physiology*
Ribosomal Proteins/genetics
Ribosomal Proteins/physiology*
Ribosomes/genetics
Ribosomes/physiology*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Yeasts/genetics
Yeasts/physiology*
Find related publications in this database (Keywords)
translational control