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Forschungsdatenbank PMU-SQQUID

Ribosomal proteins Rpl10 and Rps6 are potent regulators of yeast replicative life span.
Chiocchetti, A; Zhou, J; Zhu, H; Karl, T; Haubenreisser, O; Rinnerthaler, M; Heeren, G; Oender, K; Bauer, J; Hintner, H; Breitenbach, M; Breitenbach-Koller, L;
EXP GERONTOL. 2007; 42(4): 275-286.
Originalarbeiten (Zeitschrift)


Bauer Johann
Heeren Gino
Hintner Helmut
Önder Kamil


The yeast ribosome is composed of two subunits, the large 605 subunit (LSU) and the small 40S subunit (SSU) and harbors 78 ribosomal proteins (RPs), 59 of which are encoded by duplicate genes. Recently, deletions of the LSU paralogs RPL31A and RPL6B were found to increase significantly yeast replicative life span (RLS). RPs Rp110 and Rps6 are known translational regulators. Here, we report that heterozygosity for rpl10 Delta but not for rpl25 Delta, both LSU single copy RP genes, increased RLS by 24%. Deletion of the SSU RPS6B paralog, but not of the RPS6A paralog increased replicative life span robustly by 45%, while deletion of both the SSU RPS18A, and RPS18B paralogs increased RLS moderately, but significantly by 15%. Altering the gene dosage of RPL10 reduced the translating ribosome population, whereas deletion of the RPS6A, RPS6B, RPS18A, and RPS18B paralogs produced a large shift in free ribosomal subunit stoichiometry. We observed a reduction in growth rate in all deletion strains and reduced cell size in the SSU RPS6B, RPS6A, and RPS18B deletion strains. Thus, reduction of gene dosage of RP genes belonging to both the 605 and the 405 subunit affect lifespan, possibly altering the aging process by modulation of translation. (c) 2006 Elsevier Inc. All rights reserved.

Useful keywords (using NLM MeSH Indexing)

Cell Count

Cell Division/genetics

Cell Size

Gene Deletion

Gene Expression Regulation, Fungal/genetics

Genes, Fungal/genetics


Models, Genetic

Protein Biosynthesis/genetics

Ribosomal Protein S6/genetics*

Ribosomal Proteins/genetics*


Saccharomyces cerevisiae/genetics*

Saccharomyces cerevisiae/growth*


Saccharomyces cerevisiae Proteins/genetics*

Find related publications in this database (Keywords)

yeast replicative aging
translational capacity
ribosomal protein