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Forschungsdatenbank PMU-SQQUID

H2O2-dependent translocation of TCTP into the nucleus enables its interaction with VDR in human keratinocytes: TCTP as a further module in calcitriol signalling.
Rid, R; Onder, K; Trost, A; Bauer, J; Hintner, H; Ritter, M; Jakab, M; Costa, I; Reischl, W; Richter, K; MacDonald, S; Jendrach, M; Bereiter-Hahn, J; Breitenbach, M;
J STEROID BIOCHEM. 2010; 118(1-2): 29-40.
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Bauer Johann
Hintner Helmut
Jakab Martin
Önder Kamil
Rid Raphaela
Ritter Markus
Zurl Andrea

Abstract

Translationally controlled tumour protein (TCTP) is an evolutionarily highly conserved molecule implicated in many processes related to cell cycle progression, proliferation and growth, to the protection against harmful conditions including apoptosis and to the human allergic response. We are showing here that after application of mild oxidative stress, human TCTP relocates from the cytoplasm to the nuclei of HaCaT keratinocytes where it directly associates with the ligand-binding domain of endogenous vitamin D(3) receptor (VDR) through its helical domain 2 (AA 71-132). Interestingly, the latter harbours a putative nuclear hormone receptor coregulatory LxxLL-like motif which seems to be involved in the interaction. Moreover, we demonstrate that VDR transcriptionally induces the expression of TCTP by binding to a previously unknown VDR response element within the TCTP promotor. Conversely, ectopically overexpressed TCTP downregulates the amount of VDR on both mRNA as well as protein level. These data, to conclude, suggest a kind of feedback regulation between TCTP and VDR to regulate a variety of (Ca(2+) dependent) cellular effects and in this way further underscore the physiological relevance of this novel protein-protein interaction.


Useful keywords (using NLM MeSH Indexing)

Active Transport, Cell Nucleus/drug effects

Active Transport, Cell Nucleus/physiology

Calcitriol/pharmacology

Calcitriol/physiology*

Cell Line, Transformed

Cell Nucleus/metabolism*

Cytoplasm/metabolism

DNA/metabolism

Electrophoretic Mobility Shift Assay

Feedback, Physiological/physiology

Fluorescence Resonance Energy Transfer

Gene Expression/genetics

Gene Expression Regulation, Neoplastic/physiology

Humans

Hydrogen Peroxide/pharmacology*

Immunoprecipitation

Keratinocytes/drug effects

Keratinocytes/metabolism*

Oxidative Stress/drug effects

Oxidative Stress/physiology

Promoter Regions, Genetic/genetics

Protein Binding/drug effects

Protein Binding/physiology

Protein Interaction Domains and Motifs/physiology

Receptors, Calcitriol/genetics

Receptors, Calcitriol/metabolism*

Recombinant Proteins/metabolism

Signal Transduction/drug effects

Signal Transduction/physiology*

Tumor Markers, Biological/genetics

Tumor Markers, Biological/metabolism*

Two-Hybrid System Techniques

Vitamin D Response Element/genetics


Find related publications in this database (Keywords)

TCTP
VDR
Yeast two-hybrid
Protein-protein interaction
Skin