PMU-Autor/inn/en
Paulmichl MarkusAbstract
The present study has been performed to test for the involvement of pertussis toxin-sensitive GTP-binding proteins (G-proteins) in the cellular transduction of hormone-induced activation of potassium channels. In Madin Darby canine kidney (MDCK) cells, a permanent cell line from dog kidney, epinephrine, acetylcholine, bradykinin, serotonin and ATP hyperpolarize the cell membrane by activation of potassium channels. In cells pretreated with pertussis toxin the hyperpolarizations elicited by either acetylcholine or serotonin are completely abolished; that following epinephrine is blunted and only transient. The hyperpolarizing effects of ATP or bradykinin are not affected by pertussis toxin. Thus, in MDCK cells both pertussis toxin-dependent and -independent mechanisms operate in parallel to enhance the potassium conductance of the cell membrane.
Useful keywords (using NLM MeSH Indexing)
Acetylcholine/pharmacology*
Adenosine Triphosphate/pharmacology*
Animals
Bradykinin/pharmacology*
Cell Line
Cell Membrane/physiology*
Epinephrine/pharmacology*
Epithelium
GTP-Binding Proteins/metabolism*
Kidney
Membrane Potentials/drug effects
Pertussis Toxin*
Serotonin/pharmacology*
Virulence Factors, Bordetella/pharmacology*