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Forschungsdatenbank PMU-SQQUID

Circadian rhythmicity of inflammatory serum parameters: a neglected issue in the search of biomarkers in multiple sclerosis.
Wipfler, P; Heikkinen, A; Harrer, A; Pilz, G; Kunz, A; Golaszewski, SM; Reuss, R; Oschmann, P; Kraus, J;
J Neurol. 2013; 260(1):221-227
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Golaszewski Stefan
Harrer Andrea
Kraus Jörg
Kunz Alexander
Pilz Georg
Wipfler Peter

Abstract

Inflammatory serum parameters are intensely investigated in the search of biomarkers for disease activity and treatment response in multiple sclerosis (MS). A reason for contradictory results might be the timing of blood collection for analyzing serum concentrations of inflammatory parameters which are subject to diurnal changes. We included 34 untreated patients with relapsing-remitting MS and 34 age- and sex-matched healthy controls. 12 MS patients showed acute disease activity in corresponding MRI scans. Blood samples were obtained at 7.00, 11.00 am, 2.30, 6.00 and 9.30 pm within 1 day. We determined serum levels of cortisol and inflammatory markers including soluble tumor necrosis factor-beta (sTNF-β), soluble TNF-Receptor-1 (sTNF-R1) and -2 (sTNF-2), soluble vascular adhesion molecule-1 (sVCAM-1) and soluble intercellular adhesion molecule-1 (sICAM-1) by ELISA. We observed significantly higher serum levels of sTNF-R1 (p < 0.001) and sTNF-R2 (p < 0.001) in the morning and a significant decline of sICAM-1 (p < 0.005) and sVCAM-1 (p < 0.001) in the afternoon in both, MS patients and healthy controls. Comparison of diurnal serum levels between MS patients with active versus with non-active disease revealed significantly higher serum levels of sVCAM-1 (p < 0.05) around noon and in the early afternoon in MS patients with active disease. A significant decline of sICAM-1 (p < 0.05) in the afternoon was seen in MS patients with active and non-active disease. Our data indicate that increased awareness of potential diurnal serum concentration changes of biomarkers can eliminate one major cause of biased data as they occur in most of the investigated immunological parameters.


Useful keywords (using NLM MeSH Indexing)

Adult

Biomarkers/blood*

Case-Control Studies

Circadian Rhythm/physiology*

Female

Humans

Hydrocortisone/blood

Inflammation/blood*

Inflammation/etiology

Intercellular Adhesion Molecule-1/blood

Magnetic Resonance Imaging

Male

Middle Aged

Multiple Sclerosis/blood*

Multiple Sclerosis/physiopathology*

Receptors, Tumor Necrosis Factor/blood

Tumor Necrosis Factor-alpha/blood

Vascular Cell Adhesion Molecule-1/blood


Find related publications in this database (Keywords)

Multiple sclerosis
Circadian rhythmicity
Inflammatory serum parameters