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Forschungsdatenbank PMU-SQQUID

In vivo ligamentogenesis in embroidered poly(lactic-co-ε-caprolactone) / polylactic acid scaffolds functionalized by fluorination and hexamethylene diisocyanate cross-linked collagen foams.
Kokozidou, M; Gögele, C; Pirrung, F; Hammer, N; Werner, C; Kohl, B; Hahn, J; Breier, A; Schröpfer, M; Meyer, M; Schulze-Tanzil, G;
Histochem Cell Biol. 2023; 159(3):275-292
Originalarbeiten (Zeitschrift)


Gögele Clemens
Kokozidou Maria
Schulze-Tanzil Gundula


Although autografts represent the gold standard for anterior cruciate ligament (ACL) reconstruction, tissue-engineered ACLs provide a prospect to minimize donor site morbidity and limited graft availability. This study characterizes the ligamentogenesis in embroidered poly(L-lactide-co-ε-caprolactone) (P(LA-CL)) / polylactic acid (PLA) constructs using a dynamic nude mice xenograft model. (P(LA-CL))/PLA scaffolds remained either untreated (co) or were functionalized by gas fluorination (F), collagen foam cross-linked with hexamethylene diisocyanate (HMDI) (coll), or F combined with the foam (F + coll). Cell-free constructs or those seeded for 1 week with lapine ACL ligamentocytes were implanted into nude mice for 12 weeks. Following explantation, cell vitality and content, histo(patho)logy of scaffolds (including organs: liver, kidney, spleen), sulphated glycosaminoglycan (sGAG) contents and biomechanical properties were assessed.Scaffolds did not affect mice weight development and organs, indicating no organ toxicity. Moreover, scaffolds maintained their size and shape and reflected a high cell viability prior to and following implantation. Coll or F + coll scaffolds seeded with cells yielded superior macroscopic properties compared to the controls. Mild signs of inflammation (foreign-body giant cells and hyperemia) were limited to scaffolds without collagen. Microscopical score values and sGAG content did not differ significantly. Although remaining stable after explantation, elastic modulus, maximum force, tensile strength and strain at F

Useful keywords (using NLM MeSH Indexing)




Mice, Nude



Tissue Engineering/methods


Find related publications in this database (Keywords)

Tissue engineering
Anterior cruciate ligament
Poly(L-lactide-co-epsilon-caprolactone) (P(LA-CL))
Polylactic acid (PLA) scaffolds
Dynamic nude mice xenograft model