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Forschungsdatenbank PMU-SQQUID

Nitric oxide suppresses growth and development in the unicellular green alga Micrasterias denticulata.
Lehner, C; Kerschbaum, HH; Lütz-Meindl, U;
J Plant Physiol. 2009; 166(2):11-27
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Lehner Christine

Abstract

Nitric oxide (NO), a key molecule in inter- and intracellular signalling, is implicated in developmental processes, host defense, and apoptosis in higher plants. We investigated the effect of NO on development in the unicellular green alga, Micrasterias denticulata, using two different NO donors, S-nitroso-N-acetyl-dl-penicillamine (SNAP) and sodium nitroprusside (SNP). Investigations at the light microsopic level revealed that both NO donors suppressed cell growth. Ultrastructural analyses were performed with SNAP- as well as SNP-treated cells and, additionally, with the control compound N-acetyl-d-penicillamine (NAP). Cells incubated with NO donors lacked a secondary wall and dictyosomal function was impaired, whereas NAP-treated cells showed no difference in development and organelle structure compared to control cells. Moreover, cisternae of the Golgi stacks were slightly involute and no vesicles were pinched off after SNAP and SNP incubation. The NO scavenger cPTIO (2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, potassium salt) abrogated the effect of SNP, thus confirming that inhibition of cell growth is due to nitric oxide. Addition of iodoacetic acid, an inhibitor of cysteine-containing enzymes, like glyceraldehyde-3-phosphate dehydrogenase (GAPDH), evoked similar effects on cell growth and secondary wall formation as obtained by treatment with NO donors. Therefore, we hypothesize that NO inhibits activity of enzymes involved in the secretory pathway, such as GAPDH, via S-nitrosylation of the cysteine residue and, consequently, modulates cell growth in M. denticulata.


Useful keywords (using NLM MeSH Indexing)

Benzoates/pharmacology

Biological Assay

Cell Wall/drug effects

Cell Wall/metabolism

Cellulase/metabolism

Chlorophyta/cytology

Chlorophyta/drug effects*

Chlorophyta/growth*

development*

Chlorophyta/ultrastructure

Cyclic N-Oxides/pharmacology

Free Radical Scavengers/pharmacology

Glyceraldehyde-3-Phosphate Dehydrogenases/antagonists*

inhibitors

Imidazoles/pharmacology

Iodoacetic Acid/pharmacology

Nitric Oxide/pharmacology*

Nitric Oxide Donors/pharmacology

Nitroprusside/pharmacology

Organelles/drug effects

Organelles/metabolism

Organelles/ultrastructure

S-Nitroso-N-Acetylpenicillamine/pharmacology

Time Factors


Find related publications in this database (Keywords)

Micrasterias denticulata
Nitric oxide
SNAP
SNP
Ultrastructure