' '
Deutsch | English    

Forschungsdatenbank PMU-SQQUID

Enhanced BMP-2-Mediated Bone Repair Using an Anisotropic Silk Fibroin Scaffold Coated with Bone-like Apatite.
Deininger, C; Wagner, A; Heimel, P; Salzer, E; Vila, XM; Weißenbacher, N; Grillari, J; Redl, H; Wichlas, F; Freude, T; Tempfer, H; Teuschl-Woller, AH; Traweger, A;
Int J Mol Sci. 2021; 23(1):
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Deininger Christian
Freude Thomas
Tempfer Herbert
Traweger Andreas
Wagner Andrea
Wichlas Florian

Abstract

The repair of large bone defects remains challenging and often requires graft material due to limited availability of autologous bone. In clinical settings, collagen sponges loaded with excessive amounts of bone morphogenetic protein 2 (rhBMP-2) are occasionally used for the treatment of bone non-unions, increasing the risk of adverse events. Therefore, strategies to reduce rhBMP-2 dosage are desirable. Silk scaffolds show great promise due to their favorable biocompatibility and their utility for various biofabrication methods. For this study, we generated silk scaffolds with axially aligned pores, which were subsequently treated with 10× simulated body fluid (SBF) to generate an apatitic calcium phosphate coating. Using a rat femoral critical sized defect model (CSD) we evaluated if the resulting scaffold allows the reduction of BMP-2 dosage to promote efficient bone repair by providing appropriate guidance cues. Highly porous, anisotropic silk scaffolds were produced, demonstrating good cytocompatibility in vitro and treatment with 10× SBF resulted in efficient surface coating. In vivo, the coated silk scaffolds loaded with a low dose of rhBMP-2 demonstrated significantly improved bone regeneration when compared to the unmineralized scaffold. Overall, our findings show that this simple and cost-efficient technique yields scaffolds that enhance rhBMP-2 mediated bone healing.


Useful keywords (using NLM MeSH Indexing)

Adenosine Triphosphate/metabolism

Animals

Anisotropy

Apatites/pharmacology*

Biomimetic Materials/pharmacology

Bone Morphogenetic Protein 2/pharmacology*

Bone Regeneration/drug effects*

Bone and Bones/drug effects

Bone and Bones/physiology*

Bone and Bones/ultrastructure

Caspase 7/metabolism

Caspases/metabolism

Femur/diagnostic imaging

Femur/drug effects

Fibroins/pharmacology*

Freezing

Humans

Male

Rats, Sprague-Dawley

Recombinant Proteins/pharmacology

Tissue Scaffolds/chemistry*

Transforming Growth Factor beta/pharmacology*

X-Ray Microtomography


Find related publications in this database (Keywords)

silk scaffold
pseudarthrosis
nonunion
critical sized defect
bone regeneration