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Forschungsdatenbank PMU-SQQUID

Hsa-miR-3651 could serve as a novel predictor for in-breast recurrence via FRMD3.
Zellinger, B; Bodenhofer, U; Engländer, IA; Kronberger, C; Grambozov, B; Ruznic, E; Stana, M; Karner, J; Fastner, G; Sotlar, K; Sedlmayer, F; Zehentmayr, F;
Breast Cancer. 2022; 29(2): 274-286.
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Fastner Gerd
Grambozov Brane
Karner Josef
Kronberger Cornelia
Sedlmayer Felix
Sotlar Karl
Stana Markus
Zehentmayr Franz
Zellinger Barbara

Abstract

BACKGROUND
MicroRNAs are small non-coding RNAs with pivotal regulatory functions in multiple cellular processes. Their significance as molecular predictors for breast cancer was demonstrated in the past 15 years. The aim of this study was to elucidate the role of hsa-miR-3651 for predicting of local control (LC) in early breast cancer.
By means of high-throughput technology, hsa-miR-3651 was found to be differentially expressed between patients who experienced local relapse compared to those without (N  =  23; p  =  0.0035). This result could be validated in an independent cohort of 87 patients using RT-qPCR (p  <  0.0005). In a second analysis step with a chip-based microarray containing 70,523 probes of potential target molecules, FERM domain protein 3 (FRMD3) was found to be the most down-regulated protein (N  =  21; p  =  0.0016). Computational analysis employing different prediction algorithms revealed FRMD3 as a likely downstream target of hsa-miR-3651 with an 8mer binding site between the two molecules. This could be validated in an independent patient set (N  =  20, p  =  0.134).
The current study revealed that hsa-miR-3651 is a predictor of LC in early breast cancer via its putative target protein FRMD3. Since microRNAs interfere in multiple pathways, the results of this hypothesis generating study may contribute to the development of tailored therapies for breast cancer in the future.


Useful keywords (using NLM MeSH Indexing)

Breast Neoplasms*/genetics

Female

Gene Expression Profiling

Gene Expression Regulation, Neoplastic

Humans

MicroRNAs*/genetics

MicroRNAs*/metabolism

Neoplasm Recurrence, Local/genetics

Oligonucleotide Array Sequence Analysis


Find related publications in this database (Keywords)

Hsa-miR-3651
FRMD3
Early breast cancer
Local control
Prediction