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Forschungsdatenbank PMU-SQQUID

Improving Human Induced Pluripotent Stem Cell-Derived Megakaryocyte Differentiation and Platelet Production.
Krisch, L; Brachtl, G; Hochmann, S; Andrade, AC; Oeller, M; Ebner-Peking, P; Schallmoser, K; Strunk, D;
Int J Mol Sci. 2021; 22(15):
Originalarbeiten (Zeitschrift)


Brachtl Gabriele
Cronemberger Andrade André
Ebner Patricia
Hochmann Sarah
Krisch Linda
Öller Michaela
Schallmoser Katharina
Strunk Dirk


Several protocols exist for generating megakaryocytes (MKs) and platelets from human induced pluripotent stem cells (hiPSCs) with limited efficiency. We observed previously that mesoderm induction improved endothelial and stromal differentiation. We, therefore, hypothesized that a protocol modification prior to hemogenic endothelial cell (HEC) differentiation will improve MK progenitor (MKP) production and increase platelet output. We further asked if basic media composition affects MK maturation. In an iterative process, we first compared two HEC induction protocols. We found significantly more HECs using the modified protocol including activin A and CHIR99021, resulting in significantly increased MKs. MKs released comparable platelet amounts irrespective of media conditions. In a final validation phase, we obtained five-fold more platelets per hiPSC with the modified protocol (235 ± 84) compared to standard conditions (51 ± 15;

Find related publications in this database (Keywords)

regenerative medicine
human induced pluripotent stem cells (hiPSC)
mesoderm induction
human platelet lysate (HPL)
advanced therapy medicinal product (ATMP)