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Forschungsdatenbank PMU-SQQUID

In Vitro Evaluation of a Nanoparticle-Based mRNA Delivery System for Cells in the Joint.
Sturm, L; Schwemberger, B; Menzel, U; Hackel, S; Albers, CE; Plank, C; Rip, J; Alini, M; Traweger, A; Grad, S; Basoli, V
BIOMEDICINES. 2021; 9(7):
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Schwemberger Bettina
Sturm Lisa
Traweger Andreas

Abstract

Biodegradable and bioresponsive polymer-based nanoparticles (NPs) can be used for oligonucleotide delivery, making them a promising candidate for mRNA-based therapeutics. In this study, we evaluated and optimized the efficiency of a cationic, hyperbranched poly(amidoamine)s-based nanoparticle system to deliver tdTomato mRNA to primary human bone marrow stromal cells (hBMSC), human synovial derived stem cells (hSDSC), bovine chondrocytes (bCH), and rat tendon derived stem/progenitor cells (rTDSPC). Transfection efficiencies varied among the cell types tested (bCH 28.4% ± 22.87, rTDSPC 18.13% ± 12.07, hBMSC 18.23% ± 14.80, hSDSC 26.63% ± 8.81) and while an increase of NPs with a constant amount of mRNA generally improved the transfection efficiency, an increase of the mRNA loading ratio (2:50, 4:50, or 6:50


Find related publications in this database (Keywords)

transfection
bioresponsive polymer-based nanoparticles
joint therapies
therapeutic mRNA
biologicals