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Forschungsdatenbank PMU-SQQUID

Self-assembly of differentiated progenitor cells facilitates spheroid human skin organoid formation and planar skin regeneration
Ebner-Peking, P; Krisch, L; Wolf, M; Hochmann, S; Hoog, A; Vari, B; Muigg, K; Poupardin, R; Scharler, C; Schmidhuber, S; Russe, E; Stachelscheid, H; Schneeberger, A; Schallmoser, K; Strunk, D
THERANOSTICS. 2021; 11(17): 8430-8447.
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Ebner Patricia
Hochmann Sarah
Hoog Anna
Krisch Linda
Muigg Katharina
Poupardin Rodolphe
Russe Elisabeth
Schallmoser Katharina
Scharler Cornelia
Strunk Dirk
Vári Balázs
Wolf Martin

Abstract

Self-assembly of solid organs from single cells would greatly expand applicability of regenerative medicine. Stem/progenitor cells can self-organize into micro-sized organ units, termed organoids, partially modelling tissue function and regeneration. Here we demonstrated 3D self-assembly of adult and induced pluripotent stem cell (iPSC)-derived fibroblasts, keratinocytes and endothelial progenitors into both, planar human skin in vivo and a novel type of spheroid-shaped skin organoids in vitro, under the aegis of human platelet lysate.


Useful keywords (using NLM MeSH Indexing)

Adult

Animals

Cell Culture Techniques/methods*

Cell Differentiation/physiology

Endothelial Cells/cytology

Endothelial Progenitor Cells/cytology

Endothelial Progenitor Cells/physiology

Female

Fibroblasts/cytology

Fibroblasts/physiology

Healthy Volunteers

Humans

Induced Pluripotent Stem Cells/metabolism

Keratinocytes/cytology

Keratinocytes/physiology

Male

Mice, Inbred NOD

Middle Aged

Organoids/cytology

Organoids/metabolism*

Regeneration/physiology

Regenerative Medicine

Skin/metabolism

Skin Physiological Phenomena/genetics*

Stem Cells/metabolism*

Transfection


Find related publications in this database (Keywords)

Skin organoids
progenitor cells
human induced pluripotent stem cells
human platelet-derived growth factors
skin regeneration