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Forschungsdatenbank PMU-SQQUID

Methyl-binding domain protein-based DNA isolation from human blood serum combines DNA analyses and serum-autoantibody testing.
Wielscher, M; Pulverer, W; Peham, J; Hofner, M; Rappaport, CF; Singer, C; Jungbauer, C; Nöhammer, C; Weinhäusel, A;
BMC Clin Pathol. 2011; 11:11
Originalarbeiten (Zeitschrift)


Jungbauer Christof


Circulating cell free DNA in serum as well as serum-autoantibodies and the serum proteome have great potential to contribute to early cancer diagnostics via non invasive blood tests. However, most DNA preparation protocols destroy the protein fraction and therefore do not allow subsequent protein analyses. In this study a novel approach based on methyl binding domain protein (MBD) is described to overcome the technical difficulties of combining DNA and protein analysis out of one single serum sample.