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Forschungsdatenbank PMU-SQQUID

Bone marrow stromal cells from MDS and AML patients show increased adipogenic potential with reduced Delta-like-1 expression.
Weickert, MT; Hecker, JS; Buck, MC; Schreck, C; Rivière, J; Schiemann, M; Schallmoser, K; Bassermann, F; Strunk, D; Oostendorp, RAJ; Götze, KS;
Sci Rep. 2021; 11(1): 5944
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Schallmoser Katharina
Strunk Dirk

Abstract

Myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML) are clonal hematopoietic stem cell disorders with a poor prognosis, especially for elderly patients. Increasing evidence suggests that alterations in the non-hematopoietic microenvironment (bone marrow niche) can contribute to or initiate malignant transformation and promote disease progression. One of the key components of the bone marrow (BM) niche are BM stromal cells (BMSC) that give rise to osteoblasts and adipocytes. It has been shown that the balance between these two cell types plays an important role in the regulation of hematopoiesis. However, data on the number of BMSC and the regulation of their differentiation balance in the context of hematopoietic malignancies is scarce. We established a stringent flow cytometric protocol for the prospective isolation of a CD73