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Forschungsdatenbank PMU-SQQUID

Defining mesenchymal stromal cell (MSC)-derived small extracellular vesicles for therapeutic applications
Witwer, KW; Van Balkom, BWM; Bruno, S; Choo, A; Dominici, M; Gimona, M; Hill, AF; De Kleijn, D; Koh, M; Lai, RC; Mitsialis, SA; Ortiz, LA; Rohde, E; Asada, T; Toh, WS; Weiss, DJ; Zheng, L; Giebel, B; Lim, SK
J EXTRACELL VESICLES. 2019; 8(1): 1609206
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Gimona Mario
Rohde Eva

Abstract

Small extracellular vesicles (sEVs) from mesenchymal stromal/stem cells (MSCs) are transiting rapidly towards clinical applications. However, discrepancies and controversies about the biology, functions, and potency of MSC-sEVs have arisen due to several factors: the diversity of MSCs and their preparation; various methods of sEV production and separation; a lack of standardized quality assurance assays; and limited reproducibility of in vitro and in vivo functional assays. To address these issues, members of four societies (SOCRATES, ISEV, ISCT and ISBT) propose specific harmonization criteria for MSC-sEVs to facilitate data sharing and comparison, which should help to advance the field towards clinical applications. Specifically, MSC-sEVs should be defined by quantifiable metrics to identify the cellular origin of the sEVs in a preparation, presence of lipid-membrane vesicles, and the degree of physical and biochemical integrity of the vesicles. For practical purposes, new MSC-sEV preparations might also be measured against a well-characterized MSC-sEV biological reference. The ultimate goal of developing these metrics is to map aspects of MSC-sEV biology and therapeutic potency onto quantifiable features of each preparation.


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MSC
sEV
Therapeutics
Definition