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Forschungsdatenbank PMU-SQQUID

3D-Embedded Cell Cultures to Study Tendon Biology.
Gehwolf, R; Spitzer, G; Wagner, A; Lehner, C; Weissenbacher, N; Tempfer, H; Traweger, A;
Methods Mol Biol. 2019; 2045:155-165
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Gehwolf Renate
Lehner Christine
Spitzer Gabriel
Tempfer Herbert
Traweger Andreas
Wagner Andrea

Abstract

Tendons harbor various cell populations, including cells displaying classical adult mesenchymal stromal cell criteria. Previous studies have shown that a tenogenic phenotype is more effectively maintained in a 3D cell culture model under mechanical load. This chapter describes a method to isolate tendon-derived cells from rat Achilles tendons and the subsequent formation of 3D-embedded cell cultures. These tendon-like constructs can then be analyzed by various means, including histology, immunohistochemistry, qPCR, or standard protein analysis techniques.