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Forschungsdatenbank PMU-SQQUID

Continuous, label-free, 96-well-based determination of cell migration using confluence measurement.
Mayr, C; Beyreis, M; Dobias, H; Gaisberger, M; Fuchs, J; Pichler, M; Ritter, M; Jakab, M; Helm, K; Neureiter, D; Kiesslich, T;
Cell Adh Migr. 2019; 13(1):76-82
Originalarbeiten (Zeitschrift)


Beyreis Marlena
Dobias Heidemarie
Gaisberger Martin
Helm Katharina
Jakab Martin
Kiesslich Tobias
Mayr Christian Reinhard
Neureiter Daniel
Ritter Markus


Cellular migration is essential in diverse physiological and pathophysiological processes. Here, we present a protocol for quantitative analysis of migration using confluence detection allowing continuous, non-endpoint measurement with minimal hands-on time under cell incubator conditions. Applicability was tested using substances which enhance (EGF) or inhibit (cytochalasin D, ouabain) migration. Using a gap-closure assay we demonstrate that automated confluence detection monitors cellular migration in the 96-well microplate format. Quantification by % confluence, % cell free-area or % confluence in cell-free area against time, allows detailed analysis of cellular migration. The study describes a practicable approach for continuous, non-endpoint measurement of migration in 96-well microplates and for detailed data analysis, which allows for medium/high-throughput analysis of cellular migration in vitro.

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