' '
Deutsch | English    

Forschungsdatenbank PMU-SQQUID

Impact of glyphosate and benzo[a]pyrene on the tolerance of mosquito larvae to chemical insecticides. Role of detoxification genes in response to xenobiotics.
Riaz, MA; Poupardin, R; Reynaud, S; Strode, C; Ranson, H; David, JP;
Aquat Toxicol. 2009; 93(1):61-69
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Poupardin Rodolphe

Abstract

The effect of exposure of Aedes aegypti larvae for 72h to sub-lethal concentrations of the herbicide glyphosate and the polycyclic aromatic hydrocarbon benzo[a]pyrene on their subsequent tolerance to the chemical insecticides imidacloprid, permethrin and propoxur, detoxification enzyme activities and transcription of detoxification genes was investigated. Bioassays revealed a significant increase in larval tolerance to imidacloprid and permethrin following exposure to benzo[a]pyrene and glyphosate. Larval tolerance to propoxur increased moderately after exposure to benzo[a]pyrene while a minor increased tolerance was observed after exposure to glyphosate. Cytochrome P450 monooxygenases activities were strongly induced in larvae exposed to benzo[a]pyrene and moderately induced in larvae exposed to imidacloprid and glyphosate. Larval glutathione S-transferases activities were strongly induced after exposure to propoxur and moderately induced after exposure to benzo[a]pyrene and glyphosate. Larval esterase activities were considerably induced after exposure to propoxur but only slightly induced by other xenobiotics. Microarray screening of 290 detoxification genes following exposure to each xenobiotic with the DNA microarray Aedes Detox Chip identified multiple detoxification and red/ox genes induced by xenobiotics and insecticides. Further transcription studies using real-time quantitative RT-PCR confirmed the induction of multiple P450 genes, 1 carboxy/cholinelesterase gene and 2 red/ox genes by insecticides and xenobiotics. Overall, this study reveals the potential of benzo[a]pyrene and glyphosate to affect the tolerance of mosquito larvae to chemical insecticides, possibly through the cross-induction of particular genes encoding detoxification enzymes.


Useful keywords (using NLM MeSH Indexing)

Aedes/enzymology

Aedes/genetics

Aedes/metabolism*

Animals

Benzo(a)pyrene/pharmacokinetics

Benzo(a)pyrene/toxicity*

Biological Assay

Cytochrome P-450 Enzyme System/genetics

Cytochrome P-450 Enzyme System/metabolism

Drug Interactions

Esterases/genetics

Esterases/metabolism

Gene Expression Regulation, Enzymologic/drug effects*

Glutathione Transferase/genetics

Glutathione Transferase/metabolism

Glycine/analogs*

derivatives*

Glycine/pharmacokinetics

Glycine/toxicity

Imidazoles/pharmacokinetics

Imidazoles/toxicity

Inactivation, Metabolic/genetics

Insecticides/pharmacokinetics*

Insecticides/toxicity

Larva/enzymology

Larva/genetics

Larva/metabolism

Neonicotinoids

Nitro Compounds/pharmacokinetics

Nitro Compounds/toxicity

Oligonucleotide Array Sequence Analysis

Permethrin/pharmacokinetics

Permethrin/toxicity

Propoxur/pharmacokinetics

Propoxur/toxicity

RNA, Messenger/biosynthesis

RNA, Messenger/genetics

Reverse Transcriptase Polymerase Chain Reaction


Find related publications in this database (Keywords)

Mosquitoes
Aedes aegypti
Induction
Resistance
Insecticides
Xenobiotics
Detoxification
Cytochrome P450 monooxygenases
Glutathione S-transferases
Esterases
Oxidative stress