' '
Deutsch | English    

Forschungsdatenbank PMU-SQQUID

Transcription profiling of eleven cytochrome P450s potentially involved in xenobiotic metabolism in the mosquito Aedes aegypti.
Poupardin, R; Riaz, MA; Vontas, J; David, JP; Reynaud, S;
Insect Mol Biol. 2010; 19(2):185-193
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Poupardin Rodolphe

Abstract

Transcription profiles of 11 Aedes aegypti P450 genes from CYP6 and CYP9 subfamilies potentially involved in xenobiotic metabolism were investigated. Many genes were preferentially transcribed in tissues classically involved in xenobiotic metabolism including midgut and Malpighian tubules. Life-stage transcription profiling revealed important variations amongst larvae, pupae, and adult males and females. Exposure of mosquito larvae to sub-lethal doses of three xenobiotics induced the transcription of several genes with an induction peak after 48 to 72 h exposure. Several CYP genes were also induced by oxidative stress and one gene strongly responded to 20-hydroxyecdysone. Overall, this study revealed that these P450s show different transcription profiles according to xenobiotic exposures, life stages or sex. Their putative chemoprotective functions are discussed.


Useful keywords (using NLM MeSH Indexing)

Aedes/genetics*

Aedes/growth*

development

Aedes/metabolism*

Animals

Base Sequence

Cytochrome P-450 Enzyme System/genetics*

Cytochrome P-450 Enzyme System/metabolism*

DNA Primers/genetics

Ecdysterone/pharmacology

Female

Gene Expression Profiling

Genes, Insect/drug effects

Insect Proteins/genetics*

Insect Proteins/metabolism*

Larva/metabolism

Male

Oxidative Stress

Pupa/metabolism

Xenobiotics/metabolism*


Find related publications in this database (Keywords)

cytochrome P450 monooxygenases
CYPs
Aedes aegypti
mosquitoes
gene induction
xenobiotics
detoxification
insecticides