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Forschungsdatenbank PMU-SQQUID

Dissecting the mechanisms responsible for the multiple insecticide resistance phenotype in Anopheles gambiae s.s., M form, from Vallée du Kou, Burkina Faso.
Kwiatkowska, RM; Platt, N; Poupardin, R; Irving, H; Dabire, RK; Mitchell, S; Jones, CM; Diabaté, A; Ranson, H; Wondji, CS;
Gene. 2013; 519(1):98-106
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Poupardin Rodolphe

Abstract

With the exception of target site mutations, insecticide resistance mechanisms in the principle malaria vector Anopheles gambiae, remains largely uncharacterized in Burkina Faso. Here we detected high prevalence of resistance in Vallée du Kou (VK) to pyrethroids, DDT and dieldrin, moderate level for carbamates and full susceptibility to organophosphates. High frequencies of L1014F kdr (75%) and Rdl (87%) mutations were observed showing strong correlation with pyrethroids/DDT and dieldrin resistance. The frequency of ace1R mutation was low even in carbamate resistant mosquitoes. Microarray analysis identified genes significantly over-transcribed in VK. These include the cytochrome P450 genes, CYP6P3 and CYP6Z2, previously associated with pyrethroid resistance. Gene Ontology (GO) enrichment analysis suggested that elevated neurotransmitter activity is associated with resistance, with the over-transcription of target site resistance genes such as acetylcholinesterase and the GABA receptor. A rhodopsin receptor gene previously associated with pyrethroid resistance in Culex pipiens pallens was also over-transcribed in VK. This study highlights the complex network of mechanisms conferring multiple resistance in malaria vectors and such information should be taken into account when designing and implementing resistance control strategies.


Useful keywords (using NLM MeSH Indexing)

Acetylcholinesterase/genetics

Acetylcholinesterase/metabolism

Animals

Anopheles/drug effects*

Anopheles/genetics*

Burkina Faso

Carbamates/pharmacology

Culex/drug effects

Culex/genetics

Cytochrome P-450 Enzyme System/genetics

Cytochrome P-450 Enzyme System/metabolism

DDT/pharmacology

Dieldrin/pharmacology

Gene Frequency

Genes, Insect*

Genotype

Insect Vectors/drug effects

Insect Vectors/genetics

Insecticide Resistance/genetics*

Insecticides/pharmacology*

Microarray Analysis

Mutation

Phenotype*

Pyrethrins/pharmacology

Receptors, GABA/genetics

Receptors, GABA/metabolism

Rhodopsin/genetics

Rhodopsin/metabolism

Up-Regulation


Find related publications in this database (Keywords)

Anopheles gambiae
Malaria
Insecticide resistance
Burkina Faso
Microarray