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Forschungsdatenbank PMU-SQQUID

Comparative analysis of response to selection with three insecticides in the dengue mosquito Aedes aegypti using mRNA sequencing.
David, JP; Faucon, F; Chandor-Proust, A; Poupardin, R; Riaz, MA; Bonin, A; Navratil, V; Reynaud, S;
BMC Genomics. 2014; 15:174
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Poupardin Rodolphe

Abstract

Mosquito control programmes using chemical insecticides are increasingly threatened by the development of resistance. Such resistance can be the consequence of changes in proteins targeted by insecticides (target site mediated resistance), increased insecticide biodegradation (metabolic resistance), altered transport, sequestration or other mechanisms. As opposed to target site resistance, other mechanisms are far from being fully understood. Indeed, insecticide selection often affects a large number of genes and various biological processes can hypothetically confer resistance. In this context, the aim of the present study was to use RNA sequencing (RNA-seq) for comparing transcription level and polymorphism variations associated with adaptation to chemical insecticides in the mosquito Aedes aegypti. Biological materials consisted of a parental susceptible strain together with three child strains selected across multiple generations with three insecticides from different classes: the pyrethroid permethrin, the neonicotinoid imidacloprid and the carbamate propoxur.


Useful keywords (using NLM MeSH Indexing)

Aedes/drug effects*

Aedes/genetics*

Animals

Chromosome Mapping

Cluster Analysis

Female

Gene Expression Profiling

Gene Expression Regulation/drug effects*

Genome

High-Throughput Nucleotide Sequencing

Inactivation, Metabolic/genetics

Insecticide Resistance/genetics*

Insecticides/pharmacology*

Mice

Molecular Sequence Annotation

Polymorphism, Genetic

Polymorphism, Single Nucleotide

RNA, Messenger/genetics*

Sequence Analysis, RNA

Transcriptome


Find related publications in this database (Keywords)

RNA sequencing
RNA-seq
Insecticide resistance
Mosquito
Dengue
Detoxification enzymes
Cytochrome P450 monooxygenase
CYP
Cuticle
Transporters