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Forschungsdatenbank PMU-SQQUID

Reliability of EEG Interactions Differs between Measures and Is Specific for Neurological Diseases.
Höller, Y; Butz, K; Thomschewski, A; Schmid, E; Uhl, A; Bathke, AC; Zimmermann, G; Tomasi, SO; Nardone, R; Staffen, W; Höller, P; Leitinger, M; Höfler, J; Kalss, G; Taylor, AC; Kuchukhidze, G; Trinka, E;
Front Hum Neurosci. 2017; 11: 350
Originalarbeiten (Zeitschrift)


Butz Kevin Holger Gerd
Höfler Julia
Höller Peter
Höller Yvonne
Kalss Gudrun
Kuchukhidze Giorgi
Leitinger Markus
Nardone Raffaele
Schmid Elisabeth
Staffen Wolfgang
Thomschewski Aljoscha
Tomasi Santino Ottavio
Trinka Eugen
Zimmermann Georg Johannes


High-throughput three-dimensional cryogenic imaging of thick biological specimens is valuable for identifying biologically- or pathologically-relevant features of interest, especially for subsequent correlative studies. Unfortunately, high-resolution imaging techniques at cryogenic conditions often require sample reduction through sequential physical milling or sectioning for sufficient penetration to generate each image of the 3-D stack. This study represents the first demonstration of using ptychographic hard X-ray tomography at cryogenic temperatures for imaging thick biological tissue in a chemically-fixed, frozen-hydrated state without heavy metal staining and organic solvents. Applied to mammalian brain, this label-free cryogenic imaging method allows visualization of myelinated axons and sub-cellular features such as age-related pigmented cellular inclusions at a spatial resolution of ~100 nanometers and thicknesses approaching 100 microns. Because our approach does not require dehydration, staining or reduction of the sample, we introduce the possibility for subsequent analysis of the same tissue using orthogonal approaches that are expected to yield direct complementary insight to the biological features of interest.

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