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Forschungsdatenbank PMU-SQQUID

An In Vitro Potency Assay for Monitoring the Immunomodulatory Potential of Stromal Cell-Derived Extracellular Vesicles.
Pachler, K; Ketterl, N; Desgeorges, A; Dunai, ZA; Laner-Plamberger, S; Streif, D; Strunk, D; Rohde, E; Gimona, M;
Int J Mol Sci. 2017; 18(7):
Originalarbeiten (Zeitschrift)


Desgeorges Alexandre
Gimona Mario
Ketterl Nina
Laner-Plamberger Sandra
Pachler Karin
Rohde Eva
Somogyi-Dunai Zsuzsanna
Streif Doris
Strunk Dirk


The regenerative and immunomodulatory activity of mesenchymal stromal cells (MSCs) is partially mediated by secreted vesicular factors. Extracellular vesicles (EVs) exocytosed by MSCs are gaining increased attention as prospective non-cellular therapeutics for a variety of diseases. However, the lack of suitable in vitro assays to monitor the therapeutic potential of EVs currently restricts their application in clinical studies. We have evaluated a dual in vitro immunomodulation potency assay that reproducibly reports the inhibitory effect of MSCs on induced T-cell proliferation and the alloantigen-driven mixed leukocyte reaction of pooled peripheral blood mononuclear cells in a dose-dependent manner. Phytohemagglutinin-stimulated T-cell proliferation was inhibited by MSC-derived EVs in a dose-dependent manner comparable to MSCs. In contrast, inhibition of alloantigen-driven mixed leukocyte reaction was only observed for MSCs, but not for EVs. Our results support the application of a cell-based in vitro potency assay for reproducibly determining the immunomodulatory potential of EVs. Validation of this assay can help establish reliable release criteria for EVs for future clinical studies.

Find related publications in this database (Keywords)

extracellular vesicles
T-cell proliferation
immune modulation
mesenchymal stromal cells
mesenchymal stem/progenitor cells