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Forschungsdatenbank PMU-SQQUID

Lymphatic and vascular markers in an optic nerve crush model in rat.
Trost, A; Bruckner, D; Kaser-Eichberger, A; Motloch, K; Bogner, B; Runge, C; Strohmaier, C; Couillard-Despres, S; Reitsamer, HA; Schroedl, F;
Exp Eye Res. 2017; 159:30-39
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Bogner Barbara
Couillard-Després Sébastien
Kaser-Eichberger Alexandra
Mayr Daniela
Reitsamer Herbert
Runge Christian
Schrödl Falk
Strohmaier Clemens
Zurl Andrea

Abstract

Only few tissues lack lymphatic supply, such as the CNS or the inner eye. However, if the scleral border is compromised due to trauma or tumor, lymphatics are detected in the eye. Since the situation in the optic nerve (ON), part of the CNS, is not clear, the aim of this study is to screen for the presence of lymphatic markers in the healthy and lesioned ON. Brown Norway rats received an unilateral optic nerve crush (ONC) with defined force, leaving the dura intact. Lesioned ONs and unlesioned contralateral controls were analyzed 7 days (n = 5) and 14 days (n = 5) after ONC, with the following markers: PDGFRb (pericyte), Iba1 (microglia), CD68 (macrophages), RECA (endothelial cell), GFAP (astrocyte) as well as LYVE-1 and podoplanin (PDPN; lymphatic markers). Rat skin sections served as positive controls and confocal microscopy in single optical section mode was used for documentation. In healthy ONs, PDGFRb is detected in vessel-like structures, which are associated to RECA positive structures. Some of these PDGFRb


Useful keywords (using NLM MeSH Indexing)

Animals

Biomarkers/metabolism

Blood Vessels/pathology*

Cell Count

Disease Models, Animal

Female

Immunohistochemistry

Lymphatic Vessels/pathology*

Male

Membrane Glycoproteins/biosynthesis*

Microscopy, Confocal

Microscopy, Immunoelectron

Optic Nerve/blood supply*

Optic Nerve Injuries/diagnosis*

Optic Nerve Injuries/metabolism

Rats

Receptors, Cell Surface/biosynthesis*


Find related publications in this database (Keywords)

Lymphatic vessels
Podoplanin
LYVE-1
PDGFRb
Optic nerve crush