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Forschungsdatenbank PMU-SQQUID

Function and activation state of platelets in vitro depend on apheresis modality.
Macher, S; Sipurzynski-Budrass, S; Rosskopf, K; Rohde, E; Griesbacher, A; Groselj-Strele, A; Lanzer, G; Schallmoser, K;
VOX SANG. 2010; 99(4): 332-340.
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Rohde Eva
Schallmoser Katharina

Abstract

BACKGROUND AND OBJECTIVES
In multicomponent collection, various blood components are prepared during one apheresis process. The aim of this prospective crossover study was to compare the function, metabolic parameters and activation state of fresh and stored platelets (PLTs) collected by two different cell separators.
Twenty-four donors underwent apheresis on each of two cell separators (Fenwal Amicus(®) and CaridianBCT Trima Accel(®)) with an interval of at least 2 months between donations. Per donation, one double dose of PLT concentrate (PC) and one unit of packed red-blood-cells were collected. In total, 48 single unit PCs were tested for pH, glucose, bicarbonate, lactate, potassium and LDH concentration during 7 days of storage. PLT function was analysed by aggregometry, rotation thrombelastometry and hypotonic shock response. The PLT surface expression of P-selectin (CD62P) and LAMP-3 (CD63) was estimated by flow cytometry.
During storage, metabolic parameters were well maintained in both groups, but levels of glucose and pH were significantly lower, while lactate and LDH were significantly higher in Amicus(®)-PCs. Amicus(®)-derived PLTs were significantly more activated as evidenced by higher CD62P and CD63 expression. In parallel, the in vitro function of Amicus(®)-PLTs was significantly reduced compared to Trima(®)-PLTs.
In multicomponent apheresis, standardized PLT collection is effective and well tolerated. The higher activation of Amicus(®)-derived PLTs may be because of the divergent centrifugation modalities during collection. Possible consequences for the clinical outcome of thrombocytopenic patients will be evaluated in further trials.


Useful keywords (using NLM MeSH Indexing)

Adult

Antigens, CD/biosynthesis

Antigens, CD63

Blood Component Removal*

Blood Donors*

Blood Platelets/cytology*

Blood Platelets/metabolism*

Cross-Over Studies

Female

Gene Expression Regulation

Humans

Male

Middle Aged

Osmotic Pressure

P-Selectin/biosynthesis

Platelet Activation*

Platelet Membrane Glycoproteins/biosynthesis

Prospective Studies

Refrigeration

Thrombelastography

Time Factors


Find related publications in this database (Keywords)

multicomponent apheresis
Fenwal Amicus (R)
CaridianBCT Trima Accel (R)
platelet function
platelet activation