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Forschungsdatenbank PMU-SQQUID

Replicative senescence-associated gene expression changes in mesenchymal stromal cells are similar under different culture conditions.
Schallmoser, K; Bartmann, C; Rohde, E; Bork, S; Guelly, C; Obenauf, AC; Reinisch, A; Horn, P; Ho, AD; Strunk, D; Wagner, W;
HAEMATOL-HEMATOL J. 2010; 95(6): 86-74.
Originalarbeiten (Zeitschrift)

PMU-Autor/inn/en

Rohde Eva
Schallmoser Katharina
Strunk Dirk

Abstract

BACKGROUND
Research on mesenchymal stromal cells has created high expectations for a variety of therapeutic applications. Extensive propagation to yield enough mesenchymal stromal cells for therapy may result in replicative senescence and thus hamper long-term functionality in vivo. Highly variable proliferation rates of mesenchymal stromal cells in the course of long-term expansions under varying culture conditions may already indicate different propensity for cellular senescence. We hypothesized that senescence-associated regulated genes differ in mesenchymal stromal cells propagated under different culture conditions.
Human bone marrow-derived mesenchymal stromal cells were cultured either by serial passaging or by a two-step protocol in three different growth conditions. Culture media were supplemented with either fetal bovine serum in varying concentrations or pooled human platelet lysate.
All mesenchymal stromal cell preparations revealed significant gene expression changes upon long-term culture. Especially genes involved in cell differentiation, apoptosis and cell death were up-regulated, whereas genes involved in mitosis and proliferation were down-regulated. Furthermore, overlapping senescence-associated gene expression changes were found in all mesenchymal stromal cell preparations.
Long-term cell growth induced similar gene expression changes in mesenchymal stromal cells independently of isolation and expansion conditions. In advance of therapeutic application, this panel of genes might offer a feasible approach to assessing mesenchymal stromal cell quality with regard to the state of replicative senescence.


Useful keywords (using NLM MeSH Indexing)

Bone Marrow Cells/cytology

Bone Marrow Cells/metabolism*

Cell Aging/physiology*

Cell Culture Techniques/methods*

Cell Differentiation/physiology

Cell Proliferation*

Cells, Cultured

Gene Expression Regulation*

Humans

Mesenchymal Stromal Cells/cytology

Mesenchymal Stromal Cells/metabolism*

Stromal Cells/cytology

Stromal Cells/metabolism


Find related publications in this database (Keywords)

mesenchymal stromal cells
fetal bovine serum
pooled human platelet lysate
replicative senescence
senescence-associated gene expression